The Na+, K+, 2Cl- transporter and gill chloride secretion in palaemonid shrimps (Decapoda, Crustacea): molecular, physiological and evolutionary patterns

• Grant number: 13/23906-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2014
• Effective date (End): February 28, 2018
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: John Campbell McNamara
• Grantee: Anieli Cristina Maraschi
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Owing to its evolutionary history, the family Palaemonidae unites extant species from widely distinct osmotic environments like marine, estuarine and fresh waters, including osmotically stable or variable habitats. These shrimps compensate physiologically for osmotic challenge at both the systemic and cellular levels, employing homeostatic mechanisms that regulate the water and electrolyte concentrations of their body fluids. The gill epithelium constitutes an important interface of compensatory ion transport, and its ionocyte membranes express ion transporters that provide transepithelial ion movements. The Na+, K+, 2Cl- cotransporter (NKCC) plays a significant role in compensatory salt uptake and secretion, also participating in cell volume regulation by non-epithelial cells. In this study, we will establish a role for the NKCC in palaemonids from the genera Macrobrachium, Palaemonetes and Palaemon that occupy different osmotic niches and exhibit distinct osmoregulatory patterns. We will examine the effect of salinity exposure on hemolymph osmolality and Cl- concentration, and on quantitative gill NKCC gene and protein expression, employing molecular and biochemical analyses, including the location of the NKCC in the ionocytes membranes by immunofluorescence microscopy. Consequent to our molecular, morphological and physiological characterization, we will examine the hypotheses of: (i) a salinity effect on the evolution of hemolymph Cl- regulatory capacity and NKCC gene and protein expression; and (ii) the existence of a phylogenetic pattern in these parameters. Employing such phylogenetic comparative methods will allow reconstruction of the evolutionary history of osmoregulation at the molecular, cellular and systemic levels in the palaemonids investigated.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MCNAMARA, JOHN CAMPBELL; FREIRE, CAROLINA ARRUDA. Strategies of Invertebrate Osmoregulation: An Evolutionary Blueprint for Transmuting into Fresh Water from the Sea. INTEGRATIVE AND COMPARATIVE BIOLOGY, v. N/A, p. 12-pg., 2022-06-07. (15/00131-3, 13/23906-5, 07/04870-9, 11/22537-0, 10/17534-0)

MARASCHI, ANIELI CRISTINA; FARIA, SAMUEL COELHO; MCNAMARA, JOHN CAMPBELL. alt transport by the gill Na+-K+-2Cl(-) symporter in palaemonid shrimps: exploring physiological, molecular and evolutionary landscape. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 257, JUL 2021. (15/00131-3, 13/23906-5)

MCNAMARA, JOHN CAMPBELL; MARASCHI, ANIELI CRISTINA; TAPELLA, FEDERICO; ROMERO, MARIA CAROLINA. Evolutionary trade-offs in osmotic and ionic regulation and expression of gill ion transporter genes in high latitude, cold clime Neotropical crabs from the ?end of the world?. Journal of Experimental Biology, v. 226, n. 5, p. 16-pg., 2023-03-01. (15/00131-3, 13/23906-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARASCHI, Anieli Cristina. The Na+,K+,2Cl- cotransporter and the gill chloride secretion in Palaemonid shrimps (Decapoda, Crustacea): molecular, physiological and evolutionary patterns.. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.