Acidulated Phosphate Fluoride Application Changes the Protein Composition of Human Acquired Enamel Pellicle

Autor(es)

Masson, N. ^{[1, 2]} ; Domingues, R. R. ^[1] ; Cury, J. A. ^[2] ; Paes Leme, A. F. ^[1]

Afiliação do(s) autor(es)

^[1] CNPEM, Brazilian Biosci Natl Lab, LNBio, Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Caries Research; v. 47, n. 3, p. 251-258, 2013.
• Citações Web of Science: 6

Resumo

We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12 exclusive to APF and 11 to phosphoric acid. APF decreased the concentration of histatin-1, but increased the concentration of S100-A9, which is confirmed by immunoblotting. The findings suggest that APF application changes the acquired enamel pellicle composition. Copyright (C) 2013 S. Karger AG, Basel (AU)

• Processo FAPESP: 09/12368-7 - Análise proteômica da película adquirida salivar formada sobre esmalte e dentina tratadas com fluoreto.
• Beneficiário: Nádia Masson
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/19278-0 - Estudo da regulação de ADAMs em câncer oral
• Beneficiário: Adriana Franco Paes Leme
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores