Comparison of growth methods and biological activities of brazilian marine Streptomyces

Autor(es)

Granato, A. C. ^[1] ; Barboza, M. ^[1] ; Romano, L. H. ^[2] ; Hernandez, I. L. C. ^[2] ; Hokka, C. O. ^[2] ; Oliveira, J. H. H. L. ^[3] ; Montenegro, R. C. ^{[4, 5]} ; de Sousa, C. P. ^[6]

Afiliação do(s) autor(es)

^[1] Univ Fed Triangulo Mineiro, Dept Engn Quim, BR-38025180 Uberaba, MG - Brazil

^[2] Univ Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Alfenas, Inst Ciencias Exatas, BR-37130000 Alfenas, MG - Brazil

^[4] Univ Fed Ceara, Expt Oncol Lab, BR-60430270 Fortaleza, CE - Brazil

^[5] Univ Fed Ceara, Inst Ciencias Mar, BR-60430270 Fortaleza, CE - Brazil

^[6] Univ Fed Sao Carlos, Lab Ensino Pesquisa & Diagnost Microbiol, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 30, n. 1, p. 125-131, JAN-MAR 2013.
• Citações Web of Science: 0

Resumo

The present work describes the study of the growth and the cytotoxic and antitumor activities of the extracts of the marine microorganisms Streptomyces acrymicini and Streptomyces cebimarensis, the latter a new strain. Both microorganisms were collected from coastal marine sediments of the north coast of São Paulo state. Growth was performed in a shaker and in a bioreactor using Gym medium and the broths of both microorganisms were extracted with ethyl acetate and n-butanol. Three extracts, two organic and one aqueous, from each microorganism were obtained and tested for cytotoxic and antitumor activity using the SF-295 (Central Nervous System), HCT-8 (Colon) cell lines, and the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The growth methods were compared and show that, although the shaker presented reasonable results, the bioreactor represents the best choice for growth of these microorganisms. The biological activity of the different extracts was evaluated and it was demonstrated that the growth methodology may influence the secondary metabolite production and the biological activity. (AU)

• Processo FAPESP: 04/15540-1 - Isolamento, purificação e caracterização de cefamicina C e ácido clavulânico de Streptomyces clavuligerus
• Beneficiário: Jaine Honorata Hortolan Luiz de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/55079-4 - Produção e purificação de ácido clavulânico, cefamicina C e outros metabolitos bioativos de Streptomyces
• Beneficiário: Carlos Osamu Hokka
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/59474-8 - Estudo teórico e experimental de antibióticos beta lactâmicos e metabólitos bioativos de estreptomicetos
• Beneficiário: Ana Claudia Granato Malpass
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado