Role of the extracellular matrix in variations of invasive pathways in lung cancers

Autor(es)

de Sa, V. K. ^[1] ; Carvalho, L. ^[2] ; Gomes, A. ^[2] ; Alarcao, A. ^[2] ; Silva, M. R. ^[2] ; Couceiro, P. ^[2] ; Sousa, V. ^[2] ; Soares, F. A. ^[3] ; Capelozzi, V. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Patol, Sao Paulo - Brazil

^[2] Univ Coimbra, Coimbra - Portugal

^[3] Hosp AC Camargo Fund Antonio Prudente, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 46, n. 1, p. 21-31, JAN 2013.
• Citações Web of Science: 7

Resumo

Among the most common features of highly invasive tumors, such as lung adenocarcinomas (AD) and squamous cell carcinomas (SqCC), is the massive degradation of the extracellular matrix. The remarkable qualitative and quantitative modifications of hyaluronidases (HAases), hyaluronan synthases (HAS), E-cadherin adhesion molecules, and the transforming growth factor β (TGF-β) may favor invasion, cellular motility, and proliferation. We examined HAase proteins (Hyal), HAS, E-cadherin, and TGF-β profiles in lung AD subtypes and SqCC obtained from smokers and non-smokers. Fifty-six patients, median age 64 years, who underwent lobectomy for AD (N = 31) and SqCC (N = 25) were included in the study. HAS-1, -2 and -3, and Hyal-1 and -3 were significantly more expressed by tumor cells than normal and stroma cells (P < 0.01). When stratified according to histologic types, HAS-3 and Hyal-1 immunoreactivity was significantly increased in tumor cells of AD (P = 0.01) and stroma of SqCC (P = 0.002), respectively. Tobacco history in patients with AD was significantly associated with increased HAS-3 immunoreactivity in tumor cells (P < 0.01). Stroma cells of SqCC from non-smokers presented a significant association with HAS-3 (P < 0.01). Hyal, HAS, E-cadherin, and TGF-β modulate a different tumor-induced invasive pathway in lung AD subgroups and SqCC. HAases in resected AD and SqCC were strongly related to the prognosis. Therefore, our findings suggest that strategies aimed at preventing high HAS-3 and Hyal-1 synthesis, or local responses to low TGF-β and E-cadherin, may have a greater impact in lung cancer prognosis. (AU)

• Processo FAPESP: 08/58557-2 - Caracterização do perfil de expressão das variantes das hialuronidases e avaliação imunoproteica do ácido hialurônico, hialuronidase, agrecan e versican como marcador do prognóstico do câncer de pulmão
• Beneficiário: Vera Luiza Capelozzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57911-7 - Caracterizacao do perfil de expressao das variantes das hialuronidases e avaliacao imunoproteica das hialuronidases como marcadores de prognostico do cancer de pulmao
• Beneficiário: Vanessa Karen de Sá
• Modalidade de apoio: Bolsas no Brasil - Doutorado