Tissue Aluminum Concentration Does Not Affect the Genomic Stability of ERBB2, C-MYC, and CCND1 Genes in Breast Cancer

Autor(es)

Rodrigues-Peres, Raquel Mary ^[1] ; Cadore, Solange ^[2] ; Febraio, Stefanny ^[2] ; Heinrich, Juliana Karina ^[1] ; Serra, Katia Piton ^[1] ; Derchain, Sophie F. M. ^[1] ; Vassallo, Jose ^[3] ; Sarian, Luis Otavio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Obstet & Gynecol, Fac Med Sci, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Analyt Chem, Inst Chem, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Dept Pathol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 154, n. 3, p. 345-351, SEP 2013.
• Citações Web of Science: 3

Resumo

It has long been hypothesized that body tissue uptake of aluminum may have biological implications in breast cancer. In vitro and in vivo studies have shown that aluminum may trigger genomic instability by interfering with DNA strands. The objective of this study was to examine the relationship between aluminum concentrations in the peripheral and central areas of breast tumors with the instability of three key genes in breast cancer, ERBB2, C-MYC, and CCND1 and aneuploidy of the chromosomes harboring these genes. Tissue samples of 118 women treated for breast cancer were obtained. Evaluation of aluminum content was carried out using graphite furnace atomic absorption spectrometry. A tissue microarray slide containing the tumor samples was used in FISH assays to assess ERBB2, C-MYC, and CCND1 expressions as well as the statuses of their respective chromosomes 17, 8, and 11. Clinicopathological data were obtained from patient's records. Aluminum levels of > 2.0 mg/kg were found in 20.3 and 22.1 % of the central and peripheral breast tumor areas, respectively. Amplification and/or aneuploid-positive statuses for ERBB2/CEP17, C-MYC/CEP8, and CCND1/CEP11 were detected in 24, 36.7, and 29.3 % of the tumors, respectively. We found that aluminum concentration was not related to these altered gene statuses. Our findings suggest that aluminum concentration does not affect genomic stability in breast tissues. Tissue microenvironment modifications, due to the presence of aluminum compounds, seem more appealing as a possible target for future studies to determine the implications of aluminum in breast carcinogenesis. (AU)

• Processo FAPESP: 08/02469-8 - Instabilidade genômica em neoplasias malignas da mama em função da presença do receptor de estrógeno e da concentração de alumínio intracelular
• Beneficiário: Luís Otávio Zanatta Sarian
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/06148-4 - Instabilidade genômica em neoplasias malignas da mama em função da presença do receptor de estrógeno e da concentração de alumínio intracelular
• Beneficiário: Raquel Mary Rodrigues Peres
• Modalidade de apoio: Bolsas no Brasil - Doutorado