Maternal protein restriction during pregnancy affects gene expression and immunolocalization of intestinal nutrient transporters in rats

Autor(es)

Pinheiro, Daniela F. ^[1] ; Pinheiro, Patricia F. F. ^[2] ; Buratini, Jr., Jose ^[1] ; Castilho, Anthony C. S. ^[1] ; Lima, Paula F. ^[1] ; Trinca, Luiza A. ^[3] ; Vicentini-Paulino, Maria de Lourdes M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Physiol, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Anat, Botucatu, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Stat, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Science; v. 125, n. 5-6, p. 281-289, SEP 2013.
• Citações Web of Science: 12

Resumo

Intrauterine dietary restriction may cause changes in the functioning of offspring organs and systems later in life, an effect known as fetal programming. The present study evaluated mRNA abundance and immunolocalization of nutrient transporters as well as enterocytes proliferation in the proximal, median and distal segments of small intestine of rats born to protein-restricted dams. Pregnant rats were fed hypoproteic (6% protein) or control (17% protein) diets, and offspring rats were evaluated at 3 and 16 weeks of age. The presence of SGLT1 (sodium-glucose co-transporter 1), GLUT2 (glucose transporter 2), PEPT1 (peptide transporter 1) and the intestinal proliferation were evaluated by immunohistochemical techniques and the abundance of specific mRNA for SGLT1, GLUT2 and PEPT1 was assessed by the real-time PCR technique. Rats born to protein-restricted dams showed higher cell proliferation in all intestinal segments and higher gene expression of SGLT1 and PEPT1 in the duodenum. Moreover, in adult animals born to protein-restricted dams the immunoreactivity of SGLT1, GLUT2 and PEPT1in the duodenum was more intense than in control rats. Taken together, the results indicate that changes in the small intestine observed in adulthood can be programmed during the gestation. In addition, they show that this response is caused by both up-regulation in transporter gene expression, a specific adaptation mechanism, and intestinal proliferation, an unspecific adaptation mechanism. (AU)

• Processo FAPESP: 10/50616-0 - Estudo da expressão e localização de transportadores de nutrientes e enzimas digestivas em ratos nascidos de mães submetidas à restrição proteica durante a gestação
• Beneficiário: Maria de Lourdes Mendes Vicentini Paulino
• Modalidade de apoio: Auxílio à Pesquisa - Regular