In vivo assessment of the human sperm acrosome reaction and the expression of glycodelin-A in human endometrium after levonorgestrel-emergency contraceptive pill administration

Autor(es)

Nascimento, Josiane A. A. do ^[1] ; Seppala, Markku ; Perdigão, Antero ; Espejo-Arce, Ximena ; Munuce, Maria José ; Hautala, Laura ; Koistinen, Riitta ; Andrade, Liliana ; Bahamondes, Luís ^[9]

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas - Brasil

^[9] Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Human Reproduction; v. 22, n. 8, p. 2190-2195, Aug. 2007.
• Área do conhecimento: Ciências da Saúde - Medicina
• Assunto(s): Dispositivos anticoncepcionais femininos Levonorgestrel Espermatozoides Reação acrossômica

Resumo

The objectives were firstly to assess acrosome reaction (AR) status of spermatozoa following uterine flushing, secondly to measure levonorgestrel (LNG) levels in serum and in uterine flushing fluid and finally to measure endometrial glycodelin-A expression after administration of LNG as a form of emergency contraception (EC). Forty-eight experiments were conducted on 15 regularly menstruating women. Four groups were formed based on different intercourse to treatment interval and treatment to recovery of spermatozoa and the biopsies. Twenty-four and forty-eight hours after treatment, there were 14.5 ± 3.9 x 106 and 17.3 ± 6.8 x 106 sperm recovered from the uterus, respectively. There were no differences between the AR rate and the endometrial glycodelin-A staining intensity in an LNG or placebo treated cycles. The LNG in uterine flushing medium represented 1.38% of the values observed in serum 24 h after the LNG intake. Twenty-four and forty-eight hours after administration of EC, neither the proportion of AR sperm, nor the glycodelin-A level was influenced by 1.5 mg of LNG. LNG did not impair the cervical mucus either because viable spermatozoa were found in the genital tract 36-60 h after coitus and 24-48 h after LNG intake. The mechanism of action of LNG as EC remains unknown. (AU)

• Processo FAPESP: 03/00547-8 - Análise da taxa de reação acrossomal em espermatozoides recuperados por técnica de lavagem do cérvix e da cavidade uterina de mulheres usuárias de anticoncepção de emergência (AE) com levonorgestrel
• Beneficiário: Josiane Aparecida Andrade Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 03/08391-7 - Avaliação das repercussões clínicas e moleculares do uso de contraceptivos que contêm somente progestogênio
• Beneficiário: Luis Guillermo Bahamondes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 03/01837-0 - Análise da taxa de reação acrossomal em espermatozoides recuperados por técnica de lavagem do cérvix e da cavidade uterina de mulheres usuárias de anticoncepção de emergência (AE) com levonorgestrel
• Beneficiário: Luis Guillermo Bahamondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular