Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo assessment of the human sperm acrosome reaction and the expression of glycodelin-A in human endometrium after levonorgestrel-emergency contraceptive pill administration

Full text
Author(s):
Nascimento, Josiane A. A. do [1] ; Seppala, Markku ; Perdigão, Antero ; Espejo-Arce, Ximena ; Munuce, Maria José ; Hautala, Laura ; Koistinen, Riitta ; Andrade, Liliana ; Bahamondes, Luís [9]
Total Authors: 9
Affiliation:
[1] Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas - Brasil
[9] Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas - Brasil
Total Affiliations: 9
Document type: Journal article
Source: Human Reproduction; v. 22, n. 8, p. 2190-2195, Aug. 2007.
Field of knowledge: Health Sciences - Medicine
Abstract

The objectives were firstly to assess acrosome reaction (AR) status of spermatozoa following uterine flushing, secondly to measure levonorgestrel (LNG) levels in serum and in uterine flushing fluid and finally to measure endometrial glycodelin-A expression after administration of LNG as a form of emergency contraception (EC). Forty-eight experiments were conducted on 15 regularly menstruating women. Four groups were formed based on different intercourse to treatment interval and treatment to recovery of spermatozoa and the biopsies. Twenty-four and forty-eight hours after treatment, there were 14.5 ± 3.9 x 106 and 17.3 ± 6.8 x 106 sperm recovered from the uterus, respectively. There were no differences between the AR rate and the endometrial glycodelin-A staining intensity in an LNG or placebo treated cycles. The LNG in uterine flushing medium represented 1.38% of the values observed in serum 24 h after the LNG intake. Twenty-four and forty-eight hours after administration of EC, neither the proportion of AR sperm, nor the glycodelin-A level was influenced by 1.5 mg of LNG. LNG did not impair the cervical mucus either because viable spermatozoa were found in the genital tract 36-60 h after coitus and 24-48 h after LNG intake. The mechanism of action of LNG as EC remains unknown. (AU)

FAPESP's process: 03/08391-7 - Evaluation of the clinical and molecular repercussions of the use of contraceptives that contain only progestogen
Grantee:Luis Guillermo Bahamondes
Support Opportunities: Research Projects - Thematic Grants