Molecular profiling of matched samples identifies biomarkers of papillary thyroid carcinoma lymph node metastasis

Autor(es)

Cerutti, Janete M. ^[1] ; Oler, Gisele ; Michaluart Júnior, Pedro ; Delcelo, Rosana ; Beaty, Robert M. ; Shoemaker, Jennifer ; Riggins, Gregory J.

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Paulo (UNIFESP). Campus São Paulo - Vila Clementino. Escola Paulista de Medicina - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Cancer Research; v. 67, n. 16, p. 7885-7892, Aug. 2007.
• Área do conhecimento: Ciências da Saúde - Medicina
• Assunto(s): Biomarcadores tumorais Metástase linfática Carcinoma papilar Neoplasias da glândula tireoide Nódulo da glândula tireoide

Resumo

Biomarkers of papillary thyroid carcinoma (PTC) metastasis can accurately identify metastatic cells and aggressive tumor behavior. To find new markers, serial analysis of gene expression (SAGE) was done on three samples from the same patient: normal thyroid tissue, primary PTC, and a PTC lymph node metastasis. This genomewide expression analysis identified 31 genes expressed in lymph node metastasis, but not in the primary tumor. Eleven genes were evaluated by quantitative real-time reverse transcription-PCR (qPCR) on independent sets of matched samples to find genes that were consistently different between the tumor and metastatic samples. LIMD2 and PTPRC (CD45) showed a statistically significant difference in expression between tumor and metastatic samples (P < 0.0045), and an additional gene (LTB) had borderline significance. PTPRC and LTB were tested by immunohistochemistry in an independent set of paired samples, with both markers showing a difference in protein expression. All 20 metastases from 6 patients showed expression in both markers, with little or no expression in primary tumor. Some of these markers could provide an improved means to detect metastatic PTC cells during initial staging of a newly diagnosed carcinoma and/or to rule out recurrence. The functional role of these genes may also provide insight into mechanisms of thyroid cancer metastasis. (AU)

• Processo FAPESP: 04/15288-0 - Tumorigênese da tiróide humana: estudo funcional de mutações novas identificadas nos genes RET e BRAF e da re-expressão dos genes NESH e TARSH em linhagens celulares derivadas de carcinomas da tiróide humana
• Beneficiário: Janete Maria Cerutti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/60330-8 - Marcadores moleculares no diagnóstico e prognóstico de pacientes com tumores da tiroide humana: transição da pesquisa básica para a clínica
• Beneficiário: Janete Maria Cerutti
• Modalidade de apoio: Auxílio à Pesquisa - Temático