Melatonin and ethanol intake exert opposite effects on circulating estradiol and progesterone and differentially regulate sex steroid receptors in the ovaries, oviducts, and uteri of adult rats

Autor(es)

Chuffa, Luiz Gustavo A. ^{[1, 2]} ; Seiva, Fabio R. F. ^[3] ; Favaro, Wagner Jose ^[2] ; Amorim, Joao Paulo A. ^{[1, 2]} ; Teixeira, Giovana R. ^[2] ; Mendes, Leonardo O. ^{[1, 2]} ; Fioruci-Fontanelli, Beatriz A. ^{[1, 2]} ; Pinheiro, Patricia Fernanda F. ^[2] ; Martinez, Marcelo ^[4] ; Martinez, Francisco Eduardo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Programa Posgrad Biol Celular & Estrutural, Inst Biol, BR-13083863 Campinas, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Dept Anat, Inst Biociencias, BR-18618000 Botucatu, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Quim & Bioquim, Inst Biociencias, BR-18618000 Botucatu, SP - Brazil

^[4] UFSCar Univ Fed Sao Carlos, Dept Morfol & Patol, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTIVE TOXICOLOGY; v. 39, p. 40-49, AUG 2013.
• Citações Web of Science: 23

Resumo

Chronic ethanol intake is associated with sex hormone disturbances, and it is well known that melatonin plays a key role in regulating several reproductive processes. We report the effects of ethanol intake and melatonin treatment (at doses of 100 mu g/100g BW/day) on sex hormones and steroid receptors in the ovaries, oviducts and uteri of ethanol-preferring rats. After 150 days of treatment, animals were euthanized, and tissue samples were harvested to evaluate androgen, estrogen, progesterone and melatonin receptor subunits (AR, ER-alpha and ER-beta, PRA, PRB and MT1R, respectively). Melatonin decreased estradiol (E2) and increased progesterone (P4) and 6-sulfatoxymelatonin (6-STM), while an ethanol-melatonin combination reduced both P4 and E2. Ovarian AR was not influenced by either treatment, and oviduct AR was reduced after ethanol-melatonin combination: Oviduct ER-alpha, ER-beta and uterine ER-beta were downregulated by either ethanol or melatonin. Conversely, ovarian PRA and PRB were positively regulated by ethanol and ethanol-melatonin combination, whereas PRA was down-regulated in the uterus and oviduct after ethanol consumption. MT1R was increased in ovaries and uteri of melatonin-treated rats. Ethanol and melatonin exert opposite effects on E2 and P4, and they differentially regulate the expression of sex steroid receptors in female reproductive tissues. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/59967-7 - Ação da melatonina sobre os receptores estrogênicos ER alfa e ER beta e progestagênicos PRA e PRB nos órgãos genitais femininos internos e o estresse oxidativo nos ovários de ratas adultas UChB (consumidoras voluntárias de etanol a 10%)
• Beneficiário: Luiz Gustavo de Almeida Chuffa
• Modalidade de apoio: Bolsas no Brasil - Doutorado