Impact of diabetes mellitus and metabolic control on bone healing around osseointegrated implants: removal torque and histomorphometric analysis in rats

Autor(es)

de Molon, Rafael Scaf ^[1] ; Najarro, Juliana Aparecida ^[1] ; Morais-Camillo, Dearo ^[1] ; Arruda Verzola, Mario Henrique ^[1] ; Faeda, Rafael Silveira ^[1] ; Pepato, Maria Teresa ^[2] ; Marcantonio, Jr., Elcio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Diag & Surg, BR-14801903 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, UNESP, Dept Clin Anal, Sch Pharmaceut Sci, BR-14801903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Oral Implants Research; v. 24, n. 7, p. 831-837, JUL 2013.
• Citações Web of Science: 39

Resumo

Objectives To evaluate bone healing around dental implants with established osseointegration in experimental diabetes mellitus (DM) and insulin therapy by histomorphometric and removal torque analysis in a rat model. Materials and methods A total of 80 male Wistar rats received a titanium implant in the tibiae proximal methaphysis. After a healing period of 60days, the rats were divided into four groups of 20 animals each: a 2-month control group, sacrificed at time (group A), a diabetic group (group D), an insulin group (group I), and a 4-month control group (group C), subdivided half for removal torque and half for histomorphometric analysis. In the D and I groups the DM was induced by a single injection of 40mg/kg body weight streptozotocin (STZ). Two days after DM induction, group I received subcutaneous doses of insulin twice a day, during 2months. Groups C and D received only saline. Two months after induction of DM, the animals of groups D, C and I were sacrificed. The plasmatic levels of glucose (GPL) were monitored throughout the experiment. Evaluation of the percentages of bone-to-implant contact and bone area within the limits of the implant threads was done by histomorphometric and mechanical torque analysis. Data were analyzed by anova at significant level of 5%. Results The GPL were within normal range for groups A, C and I and higher for group D. The means and standard deviations (SD) for histomorphometric bone area showed significant difference between group D (69.34 +/- 5.00%) and groups C (78.20 +/- 4.88%) and I (79.63 +/- 4.97%). Related to bone-to-implant contact there were no significant difference between the groups D (60.81+6.83%), C (63.37+5.88%) and I (66.97+4.13%). The means and SD for removal torque showed that group D (12.91 +/- 2.51Ncm) was statistically lower than group I (17.10 +/- 3.06Ncm) and C (16.95 +/- 5.39Ncm). Conclusions Diabetes mellitus impaired the bone healing around dental implants with established osseointegration because the results presented a lower percentage of bone area in group D in relation to groups C and I resulting in a lowest torque values for implant removal. Moreover, insulin therapy prevents the occurrence of bone abnormalities found in diabetic animals and osseointegration was not compromised. (AU)

• Processo FAPESP: 04/07873-0 - 1. Eficácia da textura da imagem radiográfica digital direta, em implantes com osseointegração estabelecida, em ratos com diabetes mellitus e insulinoterapia. 2. Validação da textura de imagens tomográficas do seio maxilar, enxertado com osso autógeno e com vidro bioativo, por meio de análise histomorfométrica
• Beneficiário: Gulnara Scaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular