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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of diabetes mellitus and metabolic control on bone healing around osseointegrated implants: removal torque and histomorphometric analysis in rats

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Author(s):
de Molon, Rafael Scaf [1] ; Najarro, Juliana Aparecida [1] ; Morais-Camillo, Dearo [1] ; Arruda Verzola, Mario Henrique [1] ; Faeda, Rafael Silveira [1] ; Pepato, Maria Teresa [2] ; Marcantonio, Jr., Elcio [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Diag & Surg, BR-14801903 Sao Paulo - Brazil
[2] Univ Estadual Paulista, UNESP, Dept Clin Anal, Sch Pharmaceut Sci, BR-14801903 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Clinical Oral Implants Research; v. 24, n. 7, p. 831-837, JUL 2013.
Web of Science Citations: 39
Abstract

Objectives To evaluate bone healing around dental implants with established osseointegration in experimental diabetes mellitus (DM) and insulin therapy by histomorphometric and removal torque analysis in a rat model. Materials and methods A total of 80 male Wistar rats received a titanium implant in the tibiae proximal methaphysis. After a healing period of 60days, the rats were divided into four groups of 20 animals each: a 2-month control group, sacrificed at time (group A), a diabetic group (group D), an insulin group (group I), and a 4-month control group (group C), subdivided half for removal torque and half for histomorphometric analysis. In the D and I groups the DM was induced by a single injection of 40mg/kg body weight streptozotocin (STZ). Two days after DM induction, group I received subcutaneous doses of insulin twice a day, during 2months. Groups C and D received only saline. Two months after induction of DM, the animals of groups D, C and I were sacrificed. The plasmatic levels of glucose (GPL) were monitored throughout the experiment. Evaluation of the percentages of bone-to-implant contact and bone area within the limits of the implant threads was done by histomorphometric and mechanical torque analysis. Data were analyzed by anova at significant level of 5%. Results The GPL were within normal range for groups A, C and I and higher for group D. The means and standard deviations (SD) for histomorphometric bone area showed significant difference between group D (69.34 +/- 5.00%) and groups C (78.20 +/- 4.88%) and I (79.63 +/- 4.97%). Related to bone-to-implant contact there were no significant difference between the groups D (60.81+6.83%), C (63.37+5.88%) and I (66.97+4.13%). The means and SD for removal torque showed that group D (12.91 +/- 2.51Ncm) was statistically lower than group I (17.10 +/- 3.06Ncm) and C (16.95 +/- 5.39Ncm). Conclusions Diabetes mellitus impaired the bone healing around dental implants with established osseointegration because the results presented a lower percentage of bone area in group D in relation to groups C and I resulting in a lowest torque values for implant removal. Moreover, insulin therapy prevents the occurrence of bone abnormalities found in diabetic animals and osseointegration was not compromised. (AU)