Transcriptional changes in the expression of chemokines related to natural killer and T-regulatory cells in patients with deep infiltrative endometriosis

Autor(es)

Bellelis, Patrick ^[1] ; Barbeiro, Denise Frediani ^[2] ; Rizzo, Luiz Vicente ^[3] ; Baracat, Edmund Chada ^[1] ; Abrao, Mauricio Simoes ^[1] ; Podgaec, Sergio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Obstet & Gynecol, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Med Investigat Lab LIM 51, BR-05508 Sao Paulo - Brazil

^[3] Albert Einstein Israeli Hosp, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Fertility and Sterility; v. 99, n. 7, p. 1987-1993, JUN 2013.
• Citações Web of Science: 28

Resumo

Objective: To evaluate the expression of chemokines that regulate natural killer (NK) and T-regulatory (T-reg) cell activity in eutopic and ectopic endometrial tissue samples from endometriosis patients. Design: Case-control study (Canadian Task Force classification II-2). Setting: Tertiary referral hospital. Patient(s): Sixty-four consecutive patients with and without endometriosis. Intervention(s): After videolaparoscopy, patients were divided into three groups: bowel endometriosis (n = 22), retrocervical endometriosis (n = 10), and endometriosis-free women (n = 32). Main Outcome Measure(s): Gene expression of the chemokines that regulate NK (CXCL9, CXCL10, CXCL11, CXCL12, XCL1, and CX3CL1) and T-reg cell activity (CCL17 and CCL21) evaluated by real-time polymerase chain reaction. Result(s): Of the chemokines associated with NK cells, CX3CL1 and CXCL12 expression was statistically significantly greater in the foci of endometriosis compared with the eutopic endometrium in patients and controls. From the chemokines associated with T-reg cells, CCL17 expression was statistically significantly greater in the eutopic endometrium of the patients with rectosigmoid endometriosis compared with the foci of endometriosis or eutopic endometrium of the patients with retrocervical endometriosis or the disease-free women. Conclusion(s): Both T-reg and NK cells mediate inflammatory response and may play a fundamental role in endometriosis by causing an impaired clearing of endometrial cells. Establishing how CCL17, CXCL12, and CX3CL1 modulate this response is essential to understanding inflammatory responses in endometriosis. (Fertil Steril (R) 2013; 99: 1987-93. (C) 2013 by American Society for Reproductive Medicine.) (AU)

• Processo FAPESP: 10/00475-0 - A expressão de quimiocinas regulatórias das células natural killer e T-reguladoras em pacientes com endometriose
• Beneficiário: Sérgio Podgaec
• Modalidade de apoio: Auxílio à Pesquisa - Regular