Assessment of post-thawed ram sperm viability after incubation with seminal plasma

Autor(es)

Rovegno, Mariana ^[1] ; Feitosa, Weber Beringui ^[1] ; Rocha, Andre Monteiro ^[1] ; Mendes, Camilla Mota ^[1] ; Visintin, Jose Antonio ^[1] ; Ortiz D'Avila Assumpcao, Mayra Elena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELL AND TISSUE BANKING; v. 14, n. 2, p. 333-339, JUN 2013.
• Citações Web of Science: 10

Resumo

A suggested alternative to improve post-thawed ram semen quality is the addition of seminal plasma (SP). This is thought to be capable of improving sperm resistance to thermal shock, reverting cryocapacitation and helping sperm survival. The aim of this study was to evaluate the effect of frozen-thawed ram semen incubation with SP on mitochondrial activity, acrosomal membrane integrity, necrosis and apoptosis. Frozen/thawed semen was divided into two groups: the SP Group and the control group. After 0, 30 and 60 min, fluorescent probes were added to aliquots from each treatment group and evaluated using flow cytometry. There was no difference between treatment groups in almost all viability parameters evaluated, with exception of the apoptosis, which was found increased in SP group. The increase in incubation period resulted in a decreased percentage of sperm with high mitochondrial membrane potential and acrosomal membrane integrity and an increased percentage of necrotic and apoptotic sperm cells. In conclusion, the present study showed that addition of seminal plasma after thawing cryopreserved ram sperm had no identifiable beneficial effect on sperm quality. (AU)

• Processo FAPESP: 03/07456-8 - Estudo da integracao do dna exogeno em espermatozoides de bovinos.
• Beneficiário: Mayra Elena Ortiz D' Avila Assumpcao
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/55256-3 - Uso de sondas fluorescentes na avaliacao da viabilidade do semen descongelado acrescido de plasma seminal em ovinos.
• Beneficiário: Mariana Rovegno
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica