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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Assessment of post-thawed ram sperm viability after incubation with seminal plasma

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Author(s):
Rovegno, Mariana [1] ; Feitosa, Weber Beringui [1] ; Rocha, Andre Monteiro [1] ; Mendes, Camilla Mota [1] ; Visintin, Jose Antonio [1] ; Ortiz D'Avila Assumpcao, Mayra Elena [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Coll Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: CELL AND TISSUE BANKING; v. 14, n. 2, p. 333-339, JUN 2013.
Web of Science Citations: 10
Abstract

A suggested alternative to improve post-thawed ram semen quality is the addition of seminal plasma (SP). This is thought to be capable of improving sperm resistance to thermal shock, reverting cryocapacitation and helping sperm survival. The aim of this study was to evaluate the effect of frozen-thawed ram semen incubation with SP on mitochondrial activity, acrosomal membrane integrity, necrosis and apoptosis. Frozen/thawed semen was divided into two groups: the SP Group and the control group. After 0, 30 and 60 min, fluorescent probes were added to aliquots from each treatment group and evaluated using flow cytometry. There was no difference between treatment groups in almost all viability parameters evaluated, with exception of the apoptosis, which was found increased in SP group. The increase in incubation period resulted in a decreased percentage of sperm with high mitochondrial membrane potential and acrosomal membrane integrity and an increased percentage of necrotic and apoptotic sperm cells. In conclusion, the present study showed that addition of seminal plasma after thawing cryopreserved ram sperm had no identifiable beneficial effect on sperm quality. (AU)