| Texto completo | |
| Autor(es): |
Buarque, Diego S.
[1]
;
Braz, Gloria R. C.
[2]
;
Martins, Rafael M.
[3]
;
Tanaka-Azevedo, Anita M.
[4]
;
Gomes, Cicera M.
[4]
;
Oliveira, Felipe A. A.
[1]
;
Schenkman, Sergio
[5]
;
Tanaka, Aparecida S.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, Sao Paulo - Brazil
[2] Univ Fed Rio de Janeiro, Inst Quim, Dept Biochem, Rio De Janeiro - Brazil
[3] Inst Pasteur, Biol Host Parasite Interact Unit, Paris - France
[4] Inst Butantan, Lab Herpetol, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 8, n. 5 MAY 2 2013. |
| Citações Web of Science: | 14 |
| Resumo | |
Chagas disease, or American trypanosomiasis, is a parasitic disease caused by the protozoan Trypanosoma cruzi and is transmitted by insects from the Triatominae subfamily. To identify components involved in the protozoan-vector relationship, we constructed and analyzed cDNA libraries from RNA isolated from the midguts of uninfected and T. cruzi-infected Triatoma infestans, which are major vectors of Chagas disease. We generated approximately 440 high-quality Expressed Sequence Tags (ESTs) from each T. infestans midgut cDNA library. The sequences were grouped in 380 clusters, representing an average length of 664.78 base pairs (bp). Many clusters were not classified functionally, representing unknown transcripts. Several transcripts involved in different processes (e. g., detoxification) showed differential expression in response to T. cruzi infection. Lysozyme, cathepsin D, a nitrophorin-like protein and a putative 14 kDa protein were significantly upregulated upon infection, whereas thioredoxin reductase was downregulated. In addition, we identified several transcripts related to metabolic processes or immunity with unchanged expressions, including infestin, lipocalins and defensins. We also detected ESTs encoding juvenile hormone binding protein (JHBP), which seems to be involved in insect development and could be a target in control strategies for the vector. This work demonstrates differential gene expression upon T. cruzi infection in the midgut of T. infestans. These data expand the current knowledge regarding vector-parasite interactions for Chagas disease. (AU) | |
| Processo FAPESP: | 05/03514-9 - Estudos da função fisiológica e potencial biotecnológico de inibidores de proteases e anti-hemostáticos presentes em artrópodes hematófagos |
| Beneficiário: | Aparecida Sadae Tanaka |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 12/03657-8 - Inibidores e proteases de ectoparasitas: relação de estrutura-função e identificação do papel dessas moléculas na interação de vetores de doenças e seus agentes etiológicos |
| Beneficiário: | Aparecida Sadae Tanaka |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 07/58973-3 - Estudo do perfil de genes e proteinas do intestino medio de triatoma infestans na presenca do trypanosoma cruzi. |
| Beneficiário: | Diego de Souza Buarque |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/00723-0 - Estudo da interação de três moléculas do trato digestivo do inseto Triatoma infestans com o protozoário Trypanosoma cruzi: uma visão da relação parasita - vetor da Doença de Chagas |
| Beneficiário: | Diego de Souza Buarque |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |