Calcification in vitro of biomineralizated nanohydroxyapatite/superydrophilic vertically aligned multiwalled carbon nanotube scaffolds

Autor(es)

Neves, Marcele Florencio ^[1] ; Silva, Gislene Rodrigues ^[1] ; Brazil, Tayra Rodrigues ^[1] ; Marciano, Fernanda Roberta ^[1] ; Pacheco-Soares, Cristina ^[2] ; Lobo, Anderson Oliveira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba UniVap, Lab Nanotecnol Biomed, BR-12224000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba UniVap, Lab Dinam Compartimentos Celulares, BR-12224000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 16, n. 3, p. 614-618, MAY-JUN 2013.
• Citações Web of Science: 3

Resumo

Nanocomposites based on superhydrophilic vertically aligned multi-walled carbon nanotubes (VAMWCNT-O2) and nanohydroxyapatite (nHAp) are of great interest in bone regenerative medicine. The biomineralization using simulated body fluid (SBF) has been extensively studied to evaluate the bioactivity of biomaterials. Thus, the combination of nHAp and VAMWCNT-O2 is attractive and promising. The aim of this study was to evaluate the in vitro calcification of nHAp/VAMWCNT-O2 nanocomposites before and after the period of biomineralization in SBF. In vitro calcification of the extracellular matrix (ECM) of HOB cells in culture after 24 hours was investigated through the assay of alkaline phosphatase. These promising in vitro results validate biomineralized nHAp/VAMWCNT-O2 as possible scaffolds for bone tissue regeneration. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/02159-4 - Análise da biomineralização e proliferação celular in vitro de scaffolds de nanohidroxyapatita/nanotubos de carbono verticalmente alinhados
• Beneficiário: Tayra Rodrigues Brazil
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores