| Texto completo | |
| Autor(es): |
Gasparello-Clemente, Elaine
;
Silveira, Paulo Flavio
[2]
Número total de Autores: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicon; v. 40, n. 11, p. 1617-1626, Nov. 2002. |
| Área do conhecimento: | Ciências Biológicas - Fisiologia |
| Assunto(s): | Venenos de origem animal Serpentes Bothrops Peptídeo hidrolases Fluorometria |
| Resumo | |
In the present study we examined the feasibility of using the fluorometry of naphthylamine derivatives for revealing peptidase activities in venoms of the snakes Bothrops jararaca, Bothrops alternatus, Bothrops atrox, Bothrops moojeni, Bothrops insularis, Crotalus durissus terrificus and Bitis arietans, of the scorpions Tityus serrulatus and Tityus bahiensis, and of the spiders Phoneutria nigriventer and Loxosceles intermedia. Neutral aminopeptidase (APN) and prolyl-dipeptidyl aminopeptidase IV (DPP IV) activities were presented in all snake venoms, with the highest levels in B. alternatus. Although all examined peptidase activities showed relatively low levels in arthropod venoms, basic aminopeptidase (APB) activity from P. nigriventer venom was the exception. Compared to the other peptidase activities, relatively high levels of acid aminopeptidase (APA) activity were restricted to B. arietans venom. B. arietans also exhibited a prominent content of APB activity which was lower in other venoms. Relatively low prolyl endopeptidase and proline iminopeptidase activities were, respectively, detectable only in T. bahiensis and B. insularis. Pyroglutamate aminopeptidase activity was undetectable in all venoms. All examined peptidase activities were undetectable in T serrulatus venom. In this study, the specificities of a diverse array of peptidase activities from representative venoms were demonstrated for the first time, with a description of their distribution which may contribute to guiding further investigations. The expressive difference between snake and arthropod venoms was indicated by APN and DPP IV activities while APA and APB activities distinguished the venom of B. arietans from those of Brazilian snakes. The data reflected the relatively uniform qualitative distribution of the peptidase activities investigated, together with their unequal quantitative distribution, indicating the evolutionary divergence in the processing of peptides in these different venoms and/or the different abilities of the venoms examined to hydrolyze different peptides during envenomation. (AU) | |
| Processo FAPESP: | 00/10023-8 - Estudo comparativo de peptídeos e peptidases no sistema neuroendócrino de mamífero e réptil: células do sistema imune e em venenos animais |
| Beneficiário: | Paulo Flávio Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 01/07854-8 - Distribuição de atividades aminopeptidásicas representativas em alguns tecidos periféricos relacionados ao balanço de água e eletrólitos |
| Beneficiário: | Elaine Gasparello Clemente |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |