Development of the osteoblastic phenotype in human alveolar bone-derived cells grown on a collagen type I-coated titanium surface

Autor(es)

de Assis, Adriano Freitas ; Beloti, Marcio Mateus ; Crippa, Grasiele Edilaine ; de Oliveira, Paulo Tambasco ; Morra, Marco ^[1] ; Rosa, Adalberto Luiz ^[2]

Afiliação do(s) autor(es)

^[1] Nobil Bioric Srl, Villafranca Dasti - Italy

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Cell Culture Lab, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Oral Implants Research; v. 20, n. 3, p. 240-246, Mar. 2009.
• Área do conhecimento: Ciências da Saúde - Odontologia
• Citações Web of Science: 15
• Assunto(s): Cirurgia bucomaxilofacial Implantes dentários Osteoblastos Titânio Colágeno tipo I

Resumo

The aim of this study was to evaluate the development of the osteoblastic phenotype in human alveolar bone-derived cells grown on collagen type I-coated titanium (Ti) surface (Col-Ti) obtained by plasma deposition acrylic acid grafting compared with machined Ti (M-Ti). Osteoblastic cells were cultured until subconfluence and subcultured on Col-Ti and M-Ti for periods of up to 21 days. Cultures grown on Col-Ti and M-Ti exhibited similar cell morphology. Cell adhesion, total protein content, and alkaline phosphatase (ALP) activity were not affected by Ti surface modification in all evaluated periods. Growth analyses indicated that there were significantly more cells in cultures grown on Col-Ti at day 3. Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteoprotegerin (OPG) mRNA expression of cells subcultured on Col-Ti was higher, whereas collagen type I (COL) was lower compared with M-Ti. Ti surface modification neither affected the osteocalcin (OC), ALP and receptor activator of NF-kB ligand (RANKL)mRNA expression nor the calciumcontent extracted from mineralized matrix. These results demonstrated that Col-Ti favours cell growth during the proliferative phase (day 3) and osteoblastic differentiation, as demonstrated by changes in mRNA expression profile during the matrix mineralization phase (day 14), suggesting that this Ti surface modification may affect the processes of bone healing and remodelling. (AU)

• Processo FAPESP: 04/13756-7 - Efeito do hormonio de crescimento (gh) sobre a osteogenese em cultura de osteoblastos humanos.
• Beneficiário: Grasiele Edilaine Crippa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/06840-7 - Osteogenese in vitro sobre titanio com superficie macroporosa
• Beneficiário: Márcio Mateus Beloti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/55076-0 - Osteogenese in vitro sobre vitroceramica 100% cristalina e altamente bioativa (biosilicato): efeitos do condicionamento de superficie e dos produtos de dissolucao ionica.
• Beneficiário: Paulo Tambasco de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/09767-0 - Osteogênese em titânio: avaliação in vitro dos efeitos de diferentes métodos de estimulação
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/01768-6 - Osteogenese in vitro sobre titaniorevestido com colageno.
• Beneficiário: Adriano Freitas de Assis
• Modalidade de apoio: Bolsas no Brasil - Mestrado