The effect of agitation speed, enzyme loading and substrate concentration on enzymatic hydrolysis of cellulose from brewer's spent grain

Autor(es)

Mussatto, Solange I. ^{[1, 2]} ; Dragone, Giuliano ^[2] ; Fernandes, Marcela ^[2] ; Milagres, Adriane M. F. ^[2] ; Roberto, Ines C. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Minho, Dept Engn Biol, P-4710057 Braga - Portugal

^[2] Univ Sao Paulo, Dept Biotecnol, Escola Engn Lorena, Lorena, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cellulose; v. 15, n. 5, p. 711-721, 2008.
• Área do conhecimento: Ciências Biológicas - Microbiologia
• Citações Web of Science: 41
• Assunto(s): Biomassa Celulose Hidrólise Glicose

Resumo

Brewer’s spent grain components (cellulose, hemicellulose and lignin) were fractionated in a two-step chemical pretreatment process using dilute sulfuric acid and sodium hydroxide solutions. The cellulose pulp produced was hydrolyzed with a cellulolytic complex, Celluclast 1.5 L, at 45 °C to convert the cellulose into glucose. Several conditions were examined: agitation speed (100, 150 and 200 rpm), enzyme loading (5, 25 and 45 FPU/g substrate), and substrate concentration (2, 5 and 8% w/v), according to a 23 full factorial design aiming to maximize the glucose yield. The obtained results were interpreted by analysis of variance and response surface methodology. The optimal conditions for enzymatic hydrolysis of brewer’s spent grain were identified as 100 rpm, 45 FPU/g and 2% w/v substrate. Under these conditions, a glucose yield of 93.1% and a cellulose conversion (into glucose and cellobiose) of 99.4% was achieved. The easiness of glucose release from BSG makes this substrate a raw material with great potential to be used in bioconversion processes. (AU)

• Processo FAPESP: 07/00993-9 - Obtenção de enzimas e compostos quelantes de metais produzidos por basidiomicetos e sua relação com a degradação de compostos recalcitrantes
• Beneficiário: Adriane Maria Ferreira Milagres
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/10012-7 - Separação seletiva das principais frações orgânicas de bagaço de malte visando a produção de aditivos alimentares (xilitol e ácido láctico) por processo biotecnológico
• Beneficiário: Ines Conceicao Roberto
• Modalidade de apoio: Auxílio à Pesquisa - Regular