Intravital microscopy technique to study parasite dynamics in the labyrinth layer of the mouse placenta

Autor(es)

Lima, Flavia A. ^[1] ; Gomez-Conde, Ivan ^[2] ; Videira, Paula A. ^[1] ; Marinho, Claudio R. F. ^[3] ; Olivieri, David N. ^[2] ; Tadokoro, Carlos E.

Afiliação do(s) autor(es)

^[1] Univ Nova Lisboa, Fac Ciencias Med, Dept Imunol, CEDOC, P-1200 Lisbon - Portugal

^[2] Univ Vigo, Escuela Super Ingn Informat, Vigo 32004 - Spain

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology International; v. 63, n. 1, p. 254-259, FEB 2014.
• Citações Web of Science: 5
• Assunto(s): Plasmodium chabaudi Toxoplasma gondii Malária

Resumo

Intravital imaging techniques are the best approach to investigate in situ cellular behavior under physiological conditions. Many techniques have emerged during these last few years for this purpose. We recently described an intravital imaging technique that allows for the observation of placenta physiological responses at the labyrinth layer of this tissue. This technique will be very useful to study many placental opportunistic infections and in this article we reinforce its usefulness by analyzing placental physiological entrapment of beads and parasites. In particular, our results show that small beads (1.0 mu m) or Plasmodium chabaudi-GFP-infected-Red Blood Cells (Pc-GFP-iRBCs) cannot get trapped inside small or large blood vessels of popliteal lymph nodes (PLNs). Inside the placenta, clusters of beads could only be found inside the maternal blood vessels. However, Pc-GFP-iRBCs were found inside and outside the maternal blood vessels. We observed that trophoblasts can ingest infected-Red Blood Cells (iRBCs) in vitro and immunofluorescence of placenta revealed Pc-GFP-iRBCs inside and outside the maternal blood vessels. Taken together, we conclude that fast deposition of particles inside blood vessels seems to be an intrinsic characteristic of placenta blood flow, but iRBCs could be internalized by trophoblast cells. Thus these results represent one of the many possible uses of our intravital imaging technique to address important questions inside the parasitological field. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/53889-0 - Estudo dos mecanismos imunopatológicos envolvidos na malária durante a gravidez
• Beneficiário: Cláudio Romero Farias Marinho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores