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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Sodium Fluoride Inhibits MMP-2 and MMP-9

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Autor(es):
Kato, M. T. [1] ; Bolanho, A. [1] ; Zarella, B. L. [1] ; Salo, T. [2, 3] ; Tjaderhane, L. [2, 3, 4] ; Buzalaf, M. A. R. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[2] Univ Oulu, Inst Dent, Oulu - Finland
[3] Oulu Univ Hosp, Oulu - Finland
[4] Univ Turku, Inst Dent, Turku - Finland
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF DENTAL RESEARCH; v. 93, n. 1, p. 74-77, JAN 2014.
Citações Web of Science: 35
Resumo

The importance of fluoride (F) in preventing dental caries by favorably interfering in the demineralization-remineralization processes is well-established, but its ability to inhibit matrix metalloproteinases (MMPs), which could also help to prevent dentin caries, has not been investigated. This study assessed the ability of F to inhibit salivary and purified human gelatinases MMPs-2 and -9. Saliva was collected from 10 healthy individuals. Pooled saliva was centrifuged, and supernatants were incubated for 1 hr at 37 degrees C and subjected to zymography. Sodium fluoride (50-275 ppm F) was added to the incubation buffer. The reversibility of the inhibition of MMPs-2 and -9 by NaF was tested by the addition of NaF (250-5,000 ppm F) to the incubation buffer, after which an additional incubation was performed in the absence of F. F decreased the activities of pro- and active forms of salivary and purified human MMPs in a dose-response manner. Purified gelatinases were completely inhibited by 200 ppm F (IC50 = 100 and 75 ppm F for MMPs-2 and -9, respectively), and salivary MMP-9 by 275 ppm F (IC50 = 200 ppm F). Inhibition was partially reversible at 250-1,500 ppm F, but was irreversible at 5,000 ppm F. This is the first study to describe the ability of NaF to inhibit MMPs completely. (AU)

Processo FAPESP: 07/08389-3 - Avaliação do efeito de inibidores de metaloproteinases de matriz sobre a erosão dentária: estudos in situ / ex vivo
Beneficiário:Marília Afonso Rabelo Buzalaf
Modalidade de apoio: Auxílio à Pesquisa - Regular