| Full text | |
| Author(s): |
Kato, M. T.
[1]
;
Bolanho, A.
[1]
;
Zarella, B. L.
[1]
;
Salo, T.
[2, 3]
;
Tjaderhane, L.
[2, 3, 4]
;
Buzalaf, M. A. R.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[2] Univ Oulu, Inst Dent, Oulu - Finland
[3] Oulu Univ Hosp, Oulu - Finland
[4] Univ Turku, Inst Dent, Turku - Finland
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF DENTAL RESEARCH; v. 93, n. 1, p. 74-77, JAN 2014. |
| Web of Science Citations: | 35 |
| Abstract | |
The importance of fluoride (F) in preventing dental caries by favorably interfering in the demineralization-remineralization processes is well-established, but its ability to inhibit matrix metalloproteinases (MMPs), which could also help to prevent dentin caries, has not been investigated. This study assessed the ability of F to inhibit salivary and purified human gelatinases MMPs-2 and -9. Saliva was collected from 10 healthy individuals. Pooled saliva was centrifuged, and supernatants were incubated for 1 hr at 37 degrees C and subjected to zymography. Sodium fluoride (50-275 ppm F) was added to the incubation buffer. The reversibility of the inhibition of MMPs-2 and -9 by NaF was tested by the addition of NaF (250-5,000 ppm F) to the incubation buffer, after which an additional incubation was performed in the absence of F. F decreased the activities of pro- and active forms of salivary and purified human MMPs in a dose-response manner. Purified gelatinases were completely inhibited by 200 ppm F (IC50 = 100 and 75 ppm F for MMPs-2 and -9, respectively), and salivary MMP-9 by 275 ppm F (IC50 = 200 ppm F). Inhibition was partially reversible at 250-1,500 ppm F, but was irreversible at 5,000 ppm F. This is the first study to describe the ability of NaF to inhibit MMPs completely. (AU) | |
| FAPESP's process: | 07/08389-3 - Evaluation of the effect of matrix metalloproteinases inhibitors on dental erosion: in situ / ex vivo studies |
| Grantee: | Marília Afonso Rabelo Buzalaf |
| Support Opportunities: | Regular Research Grants |