In vitro analysis of the capacity of solutions containing fluoride and/or trimetaphosphate in the remineralization of dentin and in the inhibition of the activity of dentin matrix metalloproteinases

• Grant number: 18/19505-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2019
• End date: February 28, 2021
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Alberto Carlos Botazzo Delbem
• Grantee: Gabriel Pereira Nunes
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study will be to investigate the anti-proteolytic potential against MMP-2 and MMP-9 and the ability to induce the remineralization of dentin through solutions containing different concentrations of TMP (in its cyclic form), different concentrations of F and association TMP/F. Root dentine blocks will be divided into 10 groups of experimental solutions (6mmx4mmx2mm, n = 10): 1) without F/TMP (Placebo); 2) 0.3% TMP; 3) 1% TMP; 4) 3% TMP; 5) 250 ppm F; 6) 500 ppm F; 7) 1100 ppm F; 8) 250 ppm F associated with 0.3% TMP; 9) 500 ppm F associated with 1% TMP and 10) 1100 ppm F associated with 3% TMP. For computerized microtomographic analysis, the selected root dentin blocks will be divided into 3 areas: 1-control-healthy area; 2-demineralized area - exposed to the demineralising solution for 7 days/37ºC, treated 3-demineralized area and subjected to pH cycling for 7 days at 37ºC. Next, the analysis of the mineral content of the root dentin (Micro-CT/gHAp×cm-3). will be done. The evaluation of the anti-proteolytic potential of the experimental solutions against the dentin matrix metalloproteinases (-2 and -9) will be carried out by zymographic analysis. For this analysis, the gels will be scanned and the intensity of the band formed by densitometry will be analyzed. For statistical analysis, if mineral content and zymography data present normal distribution and homogeneity, they will be submitted to ANOVA, followed by the Student-NewmanKeuls test. If the data are not homogeneous, the Kruskall-Wallis test will be used. It will be used from the statistical program Sigmaplot for Windows version 12.0 (SigmaPlot, Systat Software Incorporation, San Jose, CA), with significance at the 5% level. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NUNES, GABRIEL PEREIRA; DANELON, MARCELLE; PESSAN, JULIANO PELIM; CAPALBO, LETICIA CABRERA; NUNES JUNIOR, NILSON ANTONIO; MATOS, ADRIANA ARRUDA; SOUZA, JOSE ANTONIO SANTOS; BUZALAF, MARILIA AFONSO RABELO; DELBEM, ALBERTO CARLOS BOTAZZO. Fluoride and trimetaphosphate association as a novel approach for remineralization and antiproteolytic activity in dentin tissue. ARCHIVES OF ORAL BIOLOGY, v. 142, p. 10-pg., 2022-07-25. (18/19505-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NUNES, Gabriel Pereira. In vitro analysis of the capacity of solutions containing fluoride and/or trimetaphosphate in the remineralization of dentin and in the inhibition of the activity of dentin matrix metalloproteinases. 2021. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.