Design, synthesis and characterization of a hexapeptide bio-inspired by acetylcholinesterase and its interaction with pesticide dichlorvos

Autor(es)

dos Santos, Glauco Pilon ^[1] ; da Silva, Bianca Ferreira ^[1] ; Garrido, Saulo Santesso ^[1] ; Mascini, Marcello ^[2] ; Yamanaka, Hideko ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Chem, BR-14800900 Araraquara, SP - Brazil

^[2] Univ Teramo, Fac Biosci & Technol Food Agr & Environm, I-64023 Teramo - Italy

• Tipo de documento: Artigo Científico
• Fonte: ANALYST; v. 139, n. 1, p. 273-279, 2014.
• Citações Web of Science: 5

Resumo

This paper describes the molecular modeling design, synthesis and characterization of a new bio-inspired hexapeptide of acetylcholinesterase enzyme and its interaction with the organophosphate pesticide dichlorvos monitored by UV-Vis spectroscopy and mass spectrometry. This strategy can contribute to the development of synthetic receptors to be coupled to biosensor transducers, avoiding the issues associated with proteins such as low stability under different pH and temperature conditions and high production cost. The resulting data of this work indicate a strong interaction between the pesticide dichlorvos and the hexapeptide (NH3+-Glu-His-Gly-Gly-Pro-Ser-COO-) with a binding constant of 4.10 x 10(5) M-1 and the formation of an adduct by covalent binding on the serine residue from the hexapeptide. (AU)

• Processo FAPESP: 10/04663-6 - Imobilização de composto biomimético em eletrodo de ouro
• Beneficiário: Glauco Pilon dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado