Local inflammatory reaction induced by Scolopendra viridicornis centipede venom in mice

Autor(es)

Kimura, Louise Faggionato ^[1] ; Prezotto-Neto, Jose Pedro ^[1] ; Lins Fernandes Tavora, Bianca de Carvalho ^[1] ; Antoniazzi, Marta Maria ^[2] ; Knysak, Irene ^[3] ; Gioia Guizze, Samuel Paulo ^[3] ; Santoro, Marcelo Larami ^[4] ; Barbaro, Katia Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Butantan Inst, Cell Biol Lab, BR-05503900 Sao Paulo - Brazil

^[3] Butantan Inst, Lab Arthropods, BR-05503900 Sao Paulo - Brazil

^[4] Butantan Inst, Lab Pathophysiol, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 76, p. 239-246, DEC 15 2013.
• Citações Web of Science: 3

Resumo

Centipede envenomation is generally mild, and human victims usually manifest burning pain, erythema and edema. Despite the abundance and ubiquity of these animals, centipede venom has been poorly characterized in literature. For this reason, the aim of this work was to investigate local inflammatory features induced by Scolopendra viridicornis centipede envenomation in mice, evaluating edema formation, leukocyte infiltration, production of inflammatory mediators, and also performing histological analysis. The highest edematogenic activity induced by the venom, determined by plethysmometry, was noticed 0.5 h after injection in mice footpad. At 24 h, edema was still detected in animals that received 15 and 60 mu g of venom, and at 48 h, only in animals injected with 60 jig of venom. In relation to leukocyte count, S. viridicornis venom induced cell recruitment, mainly neutrophils and monocytes/macrophages, in all doses and time periods analyzed in comparison with PBS-injected mice. An increase in lymphocytes was detected especially between 1 and 24 h at 60 mu g dose. Besides, eosinophil recruitment was observed mainly for 15 and 60 mu g doses in early time periods. Edema formation and cell recruitment were also confirmed by histological analysis. Moreover, S. viridicornis venom stimulated the release of IL-6, MCP-1, KC, and IL-1 beta. Conversely, S. viridicornis venom did not induce the release of detectable levels of TNF-alpha. We demonstrated that the edematogenic activity induced by S. viridicornis venom was of rapid onset, and the venom stimulated secretion of pro-inflammatory mediators which contribute to the inflammatory reaction induced by S. viridicornis venom in an experimental model. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/57898-0 - Instituto Nacional de Ciência e Tecnologia em Toxinas
• Beneficiário: Osvaldo Augusto Brazil Esteves Sant'Anna
• Linha de fomento: Auxílio à Pesquisa - Temático