Morphological and molecular characterization and phylogenetic relationships of a new species of trypanosome in Tapirus terrestris (lowland tapir), Trypanosoma terrestris sp nov., from Atlantic Rainforest of southeastern Brazil

Autor(es)

Lima Acosta, Igor da Cunha ^[1] ; da Costa, Andrea Pereira ^[1] ; Nunes, Pablo Henrique ^[2] ; Naegeli Gondim, Maria Fernanda ^[3] ; Gatti, Andressa ^[4] ; Rossi, Jr., Joao Luiz ^[3] ; Gennari, Solange Maria ^[1] ; Marcili, Arlei ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med, Dept Prevent Vet Med & Anim Sci, BR-05508 Sao Paulo - Brazil

^[2] UNESP, Biosci Inst, Dept Biol, Rio Claro, SP - Brazil

^[3] Univ Vila Velha, Fac Vet Med, Vila Velha, ES - Brazil

^[4] Marcos Daniel Inst, Vitoria, ES - Spain

• Tipo de documento: Artigo Científico
• Fonte: PARASITES & VECTORS; v. 6, DEC 11 2013.
• Citações Web of Science: 5

Resumo

Background: The Lowland tapir (Tapirus terrestris) is the largest Brazilian mammal and despite being distributed in various Brazilian biomes, it is seriously endangered in the Atlantic Rainforest. These hosts were never evaluated for the presence of Trypanosoma parasites. Methods: The Lowland tapirs were captured in the Brazilian southeastern Atlantic Rainforest, Espirito Santo state. Trypanosomes were isolated by hemoculture, and the molecular phylogeny based on small subunit rDNA (SSU rDNA) and glycosomal-3-phosphate dehydrogenase (gGAPDH) gene sequences and the ultrastructural features seen via light microscopy and scanning and transmission electron microscopy are described. Results: Phylogenetic trees using combined SSU rDNA and gGAPDH data sets clustered the trypanosomes of Lowland tapirs, which were highly divergent from other trypanosome species. The phylogenetic position and morphological discontinuities, mainly in epimastigote culture forms, made it possible to classify the trypanosomes from Lowland tapirs as a separate species. Conclusions: The isolated trypanosomes from Tapirus terrestris are a new species, Trypanosoma terrestris sp. n., and were positioned in a new Trypanosoma clade, named T. terrestris clade. (AU)

• Processo FAPESP: 10/50886-7 - Isolamento e caracterização morfológica, biológica e molecular e filogeografia multigênica de espécies causadoras de Leishmaniose visceral
• Beneficiário: Arlei Marcili
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/15311-6 - Diversidade de parasitas dos gêneros Leishmania e Trypanosoma em animais silvestres de uma unidade de conservação e animais domésticos do seu entorno no Estado do Espírito Santo.
• Beneficiário: Igor da Cunha Lima Acosta
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/19853-8 - Estudo Epidemiológico e Aspectos Ecológicos da Leishmaniose Visceral Canina no Estado do Maranhão
• Beneficiário: Andréa Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado