Glucogalactan: A polysaccharide isolated from the cell-wall of Verticillium Lecanii

Autor(es)

Corradi da Silva, Maria L. ^[1] ; Exposti, Diego T. D. ^[1] ; Vasconcelos, Ana F. D. ^[1] ; Alexandre, Samara M. A. ^[1] ; Silveira, Joana L. M. ^[2] ; Ducatti, Diogo R. B. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis Quim & Biol, BR-19060900 Sao Paulo - Brazil

^[2] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carbohydrate Polymers; v. 98, n. 2, p. 1353-1359, NOV 6 2013.
• Citações Web of Science: 4

Resumo

A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and ID and 2D H-1 and C-13 NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (EaSiv fraction) was established: {[}GRAPHICS] where n approximate to 22 and m approximate to 22. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/53879-3 - Glucanas fúngicas: caracterização química e determinação de propriedades e parâmetros físico-químicos
• Beneficiário: Maria de Lourdes Corradi Custodio da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05567-0 - Polissacarídeos da biomassa do ascomiceto Verticillium lechani: extração, purificação e caracterização
• Beneficiário: Diego Tadeu Degli Exposti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica