| Full text | |
| Author(s): |
Corradi da Silva, Maria L.
[1]
;
Exposti, Diego T. D.
[1]
;
Vasconcelos, Ana F. D.
[1]
;
Alexandre, Samara M. A.
[1]
;
Silveira, Joana L. M.
[2]
;
Ducatti, Diogo R. B.
[2]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis Quim & Biol, BR-19060900 Sao Paulo - Brazil
[2] Univ Fed Parana, Dept Bioquim & Biol Mol, BR-81531980 Curitiba, Parana - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Carbohydrate Polymers; v. 98, n. 2, p. 1353-1359, NOV 6 2013. |
| Web of Science Citations: | 4 |
| Abstract | |
A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and ID and 2D H-1 and C-13 NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (EaSiv fraction) was established: {[}GRAPHICS] where n approximate to 22 and m approximate to 22. (C) 2013 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 10/05567-0 - Polysaccharides from biomass of the ascomycetous Verticillium lechani: extraction, purification and structural characterization |
| Grantee: | Diego Tadeu Degli Exposti |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |