A photoelectrocatalytic process that disinfects water contaminated with Mycobacterium kansasii and Mycobacterium avium

Autor(es)

Brugnera, Michelle Fernanda ^[1] ; Miyata, Marcelo ^[2] ; Zocolo, Guilherme Juliao ^[1] ; Fujimura Leite, Clarice Queico ^[2] ; Boldrin Zanoni, Maria Valnice ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Inst Chem Araraquara, Dept Analyt Chem, BR-14800900 Araraquara, SP - Brazil

^[2] UNESP, Fac Pharmaceut Sci Araraquara, Dept Biol Sci, BR-14801902 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WATER RESEARCH; v. 47, n. 17, p. 6596-6605, NOV 1 2013.
• Citações Web of Science: 19

Resumo

Nontuberculous mycobacteria are resistant to conventional water treatment; indeed, they have been recovered from a wide variety of environmental sources. Here, we applied the photoelectrocatalytic technique using a Ti/TiO2-Ag photoanode to inactivate mycobacteria. For a mycobacteria population of 5 x 108 CFU mL(-1), we achieved 99.9 and 99.8% inactivation of Mycobacterium kansasii and Mycobacterium avium with rate constant of 6.2 x 10(-3) and 4.2 x 10(-3) min(-1), respectively, after 240 min. We compared the proposed method with the photolytic and photocatalytic methods. Using a mycobacteria population of 7.5 x 10(4) CFU mL(-1), the proposed Ti/TiO2-Ag photoanode elicited total mycobacteria inactivation within 3 min of treatment; the presence of Ag nanoparticles in the electrode provided 1.5 larger degradation rate constant as compared with the Ti/TiO2 anode (1.75 x 10(-2) for M. kansassi and 1.98 x 10(-2) for M. avium). We monitored the degradation of the metabolites released during cellular lysis by TOG removal, sugar release, chromatography, and mass spectrometry measurements; photoelectrocatalysis and Ti/TiO2-Ag photoanodes furnished the best results. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/09403-5 - Métodos eletroanalíticos e cromatográficos aplicados na análise de micobactérias e desinfecção fotoeletrocatalítica de micobactérias e candidas utilizando eletrodos de nanotubos de Ti/TiO2 e Ti/TiO2-Ag
• Beneficiário: Michelle Fernanda Brugnera
• Modalidade de apoio: Bolsas no Brasil - Doutorado