| Full text | |
| Author(s): |
Brugnera, Michelle Fernanda
[1]
;
Miyata, Marcelo
[2]
;
Zocolo, Guilherme Juliao
[1]
;
Fujimura Leite, Clarice Queico
[2]
;
Boldrin Zanoni, Maria Valnice
[1]
Total Authors: 5
|
| Affiliation: | [1] UNESP, Inst Chem Araraquara, Dept Analyt Chem, BR-14800900 Araraquara, SP - Brazil
[2] UNESP, Fac Pharmaceut Sci Araraquara, Dept Biol Sci, BR-14801902 Araraquara, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | WATER RESEARCH; v. 47, n. 17, p. 6596-6605, NOV 1 2013. |
| Web of Science Citations: | 19 |
| Abstract | |
Nontuberculous mycobacteria are resistant to conventional water treatment; indeed, they have been recovered from a wide variety of environmental sources. Here, we applied the photoelectrocatalytic technique using a Ti/TiO2-Ag photoanode to inactivate mycobacteria. For a mycobacteria population of 5 x 108 CFU mL(-1), we achieved 99.9 and 99.8% inactivation of Mycobacterium kansasii and Mycobacterium avium with rate constant of 6.2 x 10(-3) and 4.2 x 10(-3) min(-1), respectively, after 240 min. We compared the proposed method with the photolytic and photocatalytic methods. Using a mycobacteria population of 7.5 x 10(4) CFU mL(-1), the proposed Ti/TiO2-Ag photoanode elicited total mycobacteria inactivation within 3 min of treatment; the presence of Ag nanoparticles in the electrode provided 1.5 larger degradation rate constant as compared with the Ti/TiO2 anode (1.75 x 10(-2) for M. kansassi and 1.98 x 10(-2) for M. avium). We monitored the degradation of the metabolites released during cellular lysis by TOG removal, sugar release, chromatography, and mass spectrometry measurements; photoelectrocatalysis and Ti/TiO2-Ag photoanodes furnished the best results. (C) 2013 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 09/09403-5 - Electroanalytical and chromatographic methods applied in the analysis of mycobacteria and photoeletrocatalytic disinfection of mycobacteria and candidas using electrodes of Ti/TiO2 and Ti/TiO2-Ag nanotubes |
| Grantee: | Michelle Fernanda Brugnera |
| Support Opportunities: | Scholarships in Brazil - Doctorate |