Clonal complexes 104, 109 and 113 playing a major role in the dissemination of OXA-carbapenemase-producing Acinetobacter baumannii in Southeast Brazil

Autor(es)

Climaco, Eduardo Carneiro ^[1] ; de Oliveira, Milena Locci ^[1] ; Pitondo-Silva, Andre ^[1] ; Oliveira, Murilo Gomes ^[2] ; Medeiros, Micheli ^{[3, 4]} ; Lincopan, Nilton ^{[3, 4]} ; da Costa Darini, Ana Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Juiz de Fora, Dept Ciencias Farmaceut, Fac Farm, Juiz De Fora - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 19, p. 127-133, OCT 2013.
• Citações Web of Science: 31

Resumo

Carbapenem resistance among Acinetobacter baumannii strains isolated from clinical settings in Brazil has increased dramatically in the last 10 years due to the emergence and dissemination of OXA-type carbapenemase encoding genes. This study aimed to characterize the presence of carbapenem-hydrolyzing class D beta-lactamases (CHDL)-encoding genes and clonal complexes playing a major role in the dissemination of OXA-carbapenemase-producing A. baumannii in Southeast Brazil. A total of 74 A. baumannii strains isolated from patients admitted to 4 hospitals in Southeast Brazil were analyzed. Molecular characterization of strains revealed that 67 strains carried bla(OXA-23) (72%), bla(OXA-143) (25%) or both genes (3%). PFGE analysis identified 12 PFGE clusters, grouping 26 pulsotypes. Two PFGE clusters were predominant, comprising more than 66% of OXA-producing A. baumannii isolates. Among 23 representative strains characterized by MLST-UO (Multilocus Sequence Typing Scheme - University of Oxford, http://pubmlst.org/abaumannii/), 14 different STs were identified, of which six were confirmed as novel sequence types (designated as STs 402-407). Most of these isolates belonged to clonal complexes CC104,CC109 or CC113, whereas three STs were singletons (ST339, 403 and 407). In conclusion, the presence of bla(OXA-23)-and bla(OXA-143)-like genes was not related to specific ST/CC, suggesting that the dissemination of OXA-carbapenemase-encoding genes may involve different STs, in which the spread of OXA-23-like is most likely due to mobile elements (i.e., plasmids). In this regard, CC104, CC109 and CC113 played a major role as predominant CDHL-carrying clones, instead of CC92, which was not identified. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/08210-1 - Tipagem molecular de Acinetobacter baumannii resistentes aos carbapenêmicos isoladas em Hospital Universitário
• Beneficiário: Ana Lúcia da Costa Darini
• Modalidade de apoio: Auxílio à Pesquisa - Regular