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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Clonal complexes 104, 109 and 113 playing a major role in the dissemination of OXA-carbapenemase-producing Acinetobacter baumannii in Southeast Brazil

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Author(s):
Climaco, Eduardo Carneiro [1] ; de Oliveira, Milena Locci [1] ; Pitondo-Silva, Andre [1] ; Oliveira, Murilo Gomes [2] ; Medeiros, Micheli [3, 4] ; Lincopan, Nilton [3, 4] ; da Costa Darini, Ana Lucia [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Juiz de Fora, Dept Ciencias Farmaceut, Fac Farm, Juiz De Fora - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: INFECTION GENETICS AND EVOLUTION; v. 19, p. 127-133, OCT 2013.
Web of Science Citations: 31
Abstract

Carbapenem resistance among Acinetobacter baumannii strains isolated from clinical settings in Brazil has increased dramatically in the last 10 years due to the emergence and dissemination of OXA-type carbapenemase encoding genes. This study aimed to characterize the presence of carbapenem-hydrolyzing class D beta-lactamases (CHDL)-encoding genes and clonal complexes playing a major role in the dissemination of OXA-carbapenemase-producing A. baumannii in Southeast Brazil. A total of 74 A. baumannii strains isolated from patients admitted to 4 hospitals in Southeast Brazil were analyzed. Molecular characterization of strains revealed that 67 strains carried bla(OXA-23) (72%), bla(OXA-143) (25%) or both genes (3%). PFGE analysis identified 12 PFGE clusters, grouping 26 pulsotypes. Two PFGE clusters were predominant, comprising more than 66% of OXA-producing A. baumannii isolates. Among 23 representative strains characterized by MLST-UO (Multilocus Sequence Typing Scheme - University of Oxford, http://pubmlst.org/abaumannii/), 14 different STs were identified, of which six were confirmed as novel sequence types (designated as STs 402-407). Most of these isolates belonged to clonal complexes CC104,CC109 or CC113, whereas three STs were singletons (ST339, 403 and 407). In conclusion, the presence of bla(OXA-23)-and bla(OXA-143)-like genes was not related to specific ST/CC, suggesting that the dissemination of OXA-carbapenemase-encoding genes may involve different STs, in which the spread of OXA-23-like is most likely due to mobile elements (i.e., plasmids). In this regard, CC104, CC109 and CC113 played a major role as predominant CDHL-carrying clones, instead of CC92, which was not identified. (C) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/08210-1 - Molecular typing of Acinetobacter baumannii carbapenem resistent isolated in a University Hospital
Grantee:Ana Lúcia da Costa Darini
Support Opportunities: Regular Research Grants