Kinetic studies of recombinant rabies virus glycoprotein (RVGP) cDNA transcription and mRNA translation in Drosophila melanogaster S2 cell populations

Autor(es)

Astray, R. M. ^[1] ; Jorge, S. A. C. ^[1] ; Lemos, M. A. N. ^[1] ; Yokomizo, A. Y. ^[1] ; Boldorini, V. L. L. ^[1] ; Puglia, A. L. P. ^[1] ; Ribeiro, O. G. ^[2] ; Pereira, C. A. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil

^[2] Inst Butantan, Lab Imunogenet, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cytotechnology; v. 65, n. 5, p. 829-838, OCT 2013.
• Citações Web of Science: 4

Resumo

Recombinant rabies virus glycoprotein (RVGP) was expressed in cell membranes of stably transfected Drosophila S2 cells using constitutive and inducible promoters. Although with quantitative differences of RVGP expression in both systems, the cDNA transcription, as evaluated by relative RVGP mRNA levels measured by qRT-PCR, sustained the amount of RVGP producing cells and the RVGP volumetric (I (RVGP)) productivity. At the transition to the stationary cell growth phase, once the cell culture slowed down its rate of multiplication, an accumulation of RVGP mRNA and RVGP was clearly observed in both cell populations. Nevertheless, cell cultures performed under sub-optimal temperatures indicated that an envisaged increase in the RVGP production is not only dependent on cell growth rate, but essentially on optimal cell metabolic state. (AU)

• Processo FAPESP: 11/08331-0 - Estudo da dinâmica quantitativa de transcrição de gene heterólogo em células animais transfectadas para otimização de bioprocesso
• Beneficiário: Carlos Augusto Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular