Sperm nuclear DNA fragmentation rate is associated with differential protein expression and enriched functions in human seminal plasma

Autor(es)

Intasqui, Paula ^[1] ; Camargo, Mariana ^[1] ; Del Giudice, Paula T. ^[1] ; Spaine, Deborah M. ^[1] ; Carvalho, Valdemir M. ^[2] ; Cardozo, Karina H. M. ^[2] ; Zylbersztejn, Daniel S. ^[1] ; Bertolla, Ricardo P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 Sao Paulo - Brazil

^[2] Fleury Grp, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BJU INTERNATIONAL; v. 112, n. 6, p. 835-843, OCT 2013.
• Citações Web of Science: 17

Resumo

Objective To analyse the proteomic profile of seminal plasma with the aim of identifying the proteins and post-genomic pathways associated with sperm DNA fragmentation. Materials and Methods A cross-sectional study including 89 subjects from a human reproduction service was carried out. All semen samples were assessed for sperm DNA fragmentation using a comet assay. Results from 60 sperm were analysed using Komet 6.0.1 software and the `Olive tail moment' variable was used to stratify these into low and high sperm DNA fragmentation groups. Seminal plasma proteins from the two groups were pooled and used for proteomic analysis. Quantitative data were used for functional enrichment studies. Results Seventy-two proteins were identified or quantified in seminal plasma. Of these, nine were differentially expressed in the low group and 21 in the high group. Forty-two proteins were conserved between these groups. Functional enrichment analysis indicated that sperm DNA fragmentation was related to functions such as lipoprotein particle remodelling and regulation, fatty acid binding and immune response. Proteins found exclusively in the low group may be involved in correcting spermatogenesis and/or improving sperm function. Proteins in the high group were associated with increased innate immune response, sperm motility and/or maturation and inhibition of mitochondrial apoptosis. Conclusion Protein expression and post-genomic pathways of seminal plasma differ according to the rate of sperm DNA integrity. (AU)

• Processo FAPESP: 11/00385-4 - Comparação dos perfis protéicos de plasma seminal e de espermatozóides com baixa e alta fragmentação de DNA
• Beneficiário: Paula Intasqui Lopes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica