Biochemical and anatomical responses related to the in vitro survival of the tropical bromeliad Nidularium minutum to low temperatures

Autor(es)

Carvalho, Camila Pereira ^{[1, 2]} ; Hayashi, Adriana Hissae ^[3] ; Braga, Marcia Regina ^[4] ; Nievola, Catarina Carvalho ^[1]

Afiliação do(s) autor(es)

^[1] Inst Bot, Nucleo Pesquisa Plantes Ornamentais, BR-04045972 Sao Paulo - Brazil

^[2] Inst Bot, Programa Posgrad Biodiversidade Vegetal & Meio Am, BR-04045972 Sao Paulo - Brazil

^[3] Inst Bot, Nucleo Pesquisa Anat, BR-04045972 Sao Paulo - Brazil

^[4] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, BR-04045972 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Physiology and Biochemistry; v. 71, p. 144-154, OCT 2013.
• Citações Web of Science: 14

Resumo

Nidularium minutum is a tropical bromeliad that grows in natural environment with temperatures ranging from 2 to 30 degrees C. In the present work we cultivated this species in vitro at 5,10,15, and 25 degrees C for 3 and 6 months aiming at assessing biochemical and morphological responses that allow its survival under low temperatures. No survival was observed for plants cultured constantly at 5 degrees C and the lowest biometric parameters were found for those grown at 10 degrees C. A thick aquiferous parenchyma, accumulation of reducing sugars, and increased pectin content in the cell walls were observed in plants grown at 10 and 15 degrees C when compared to those maintained at 25 degrees C. In plants cultured at 10 degrees C, leaf bleaching correlated with low chlorophyll content and lower survival rate after 6 months when compared to those grown at 15 degrees C. The best in vitro culture condition for slow growth and plant acclimatization was found to be at 15 degrees C. This probably correlated with the immediate availability of carbon to restore growth during acclimatization and also with higher root initiation under this condition. This study brings information about the responses related to functional adaptation to low temperatures in N. minutum cultured in vitro that can also be implicated in its survival under natural conditions. Additionally, it suggests the best temperature to form a minimal growth collection to be used in restocking and conservation programs for endangered tropical bromeliads. (C) 2013 Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 05/04139-7 - Carboidratos de plantas tropicais como moduladores de processos ecofisiológicos e indicadores de respostas a estresses ambientais
• Beneficiário: Marcia Regina Braga
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/16332-0 - Carboidratos de reserva e de parede celular de plantas e de fungos filamentosos: alterações em resposta a aleloquímicos e a variações ambientais
• Beneficiário: Marcia Regina Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular