| Full text | |
| Author(s): |
Carvalho, Camila Pereira
[1, 2]
;
Hayashi, Adriana Hissae
[3]
;
Braga, Marcia Regina
[4]
;
Nievola, Catarina Carvalho
[1]
Total Authors: 4
|
| Affiliation: | [1] Inst Bot, Nucleo Pesquisa Plantes Ornamentais, BR-04045972 Sao Paulo - Brazil
[2] Inst Bot, Programa Posgrad Biodiversidade Vegetal & Meio Am, BR-04045972 Sao Paulo - Brazil
[3] Inst Bot, Nucleo Pesquisa Anat, BR-04045972 Sao Paulo - Brazil
[4] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, BR-04045972 Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Plant Physiology and Biochemistry; v. 71, p. 144-154, OCT 2013. |
| Web of Science Citations: | 14 |
| Abstract | |
Nidularium minutum is a tropical bromeliad that grows in natural environment with temperatures ranging from 2 to 30 degrees C. In the present work we cultivated this species in vitro at 5,10,15, and 25 degrees C for 3 and 6 months aiming at assessing biochemical and morphological responses that allow its survival under low temperatures. No survival was observed for plants cultured constantly at 5 degrees C and the lowest biometric parameters were found for those grown at 10 degrees C. A thick aquiferous parenchyma, accumulation of reducing sugars, and increased pectin content in the cell walls were observed in plants grown at 10 and 15 degrees C when compared to those maintained at 25 degrees C. In plants cultured at 10 degrees C, leaf bleaching correlated with low chlorophyll content and lower survival rate after 6 months when compared to those grown at 15 degrees C. The best in vitro culture condition for slow growth and plant acclimatization was found to be at 15 degrees C. This probably correlated with the immediate availability of carbon to restore growth during acclimatization and also with higher root initiation under this condition. This study brings information about the responses related to functional adaptation to low temperatures in N. minutum cultured in vitro that can also be implicated in its survival under natural conditions. Additionally, it suggests the best temperature to form a minimal growth collection to be used in restocking and conservation programs for endangered tropical bromeliads. (C) 2013 Elsevier Masson SAS. All rights reserved. (AU) | |
| FAPESP's process: | 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers |
| Grantee: | Marcia Regina Braga |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/16332-0 - Storage and cell wall carbohydrates from plants and filamentous fungi: changes in response to alelochemicals and environmental conditions |
| Grantee: | Marcia Regina Braga |
| Support Opportunities: | Regular Research Grants |