Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Proteomic Analysis of Liver in Rats Chronically Exposed to Fluoride

Texto completo
Autor(es):
Barbosa da Silva Pereira, Heloisa Aparecida [1] ; Leite, Aline de Lima [1] ; Charone, Senda [1] ; Vaz Madureira Lobo, Janete Gualiume [1] ; Cestari, Tania Mary [1] ; Peres-Buzalaf, Camila [1] ; Rabelo Buzalaf, Marilia Afonso [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Biol Sci, Bauru Dent Sch, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 8, n. 9 SEP 17 2013.
Citações Web of Science: 29
Resumo

Fluoride (F) is a potent anti-cariogenic element, but when ingestion is excessive, systemic toxicity may be observed. This can occur as acute or chronic responses, depending on both the amount of F and the time of exposure. The present study identified the profile of protein expression possibly associated with F-induced chronic hepatotoxicity. Weanling male Wistar rats (three-weeks old) were divided into three groups and treated with drinking water containing 0, 5 or 50 mg/L F for 60 days (n=6/group). At this time point, serum and livers were collected for F analysis, which was done using the ion-sensitive electrode, after hexamethyldisiloxane-facilitated diffusion. Livers were also submitted to histological and proteomic analyses (2D-PAGE followed by LC-MS/MS). Western blotting was done for confirmation of the proteomic data A dose-response was observed in serum F levels. In the livers, F levels were significantly increased in the 50 mg/L F group compared to groups treated with 0 and 5 mg/L F. Liver morphometric analysis did not reveal alterations in the cellular structures and lipid droplets were present in all groups. Proteomic quantitative intensity analysis detected 33, 44, and 29 spots differentially expressed in the comparisons between control vs. 5 mg/L F, control vs. 50 mg/L F, and 5 mg/L vs. 50 mg/L F, respectively. From these, 92 proteins were successfully identified. In addition, 18, 1, and 5 protein spots were shown to be exclusive in control, 5, and 50 mg/L F, respectively. Most of proteins were related to metabolic process and pronounced alterations were seen for the high-F level group. In F-treated rats, changes in the apolipoprotein E (ApoE) and GRP-78 expression may account for the F-induced toxicity in the liver. This can contribute to understanding the molecular mechanisms underlying hepatoxicity induced by F, by indicating key-proteins that should be better addressed in future studies. (AU)

Processo FAPESP: 08/07263-9 - Análise proteômica em fígado de ratos submetidos à exposição crônica ao flúor
Beneficiário:Heloisa Aparecida Barbosa da Silva Pereira
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/06892-5 - Análise proteômica em fígado de ratos submetidos à exposição crônica ao flúor
Beneficiário:Marília Afonso Rabelo Buzalaf
Modalidade de apoio: Auxílio à Pesquisa - Regular