Prior reproductive experience alters prolactin-induced macrophage responses in pregnant rats

Autor(es)

Roth Carvalho-Freitas, Maria Isabel ^[1] ; Anselmo-Franci, Janete A. ^[2] ; Palermo-Neto, Joao ^[1] ; Felicio, Luciano F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Morphol Stomatol & Physiol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF REPRODUCTIVE IMMUNOLOGY; v. 99, n. 1-2, p. 54-61, SEP 2013.
• Citações Web of Science: 6

Resumo

Reproductive experience (i.e., pregnancy and lactation) induces physiological changes in mammals. A previous reproductive experience was recently shown to modulate the activity of dopaminergic hypothalamic systems while decreasing serum prolactin levels and oxidative burst activity in peritoneal macrophages. Dopamine receptor antagonists increase serum prolactin levels, and both prolactin and dopamine receptors may be involved in the modulation of macrophage activity, providing a means of communication between the nervous and immune systems. The present study evaluated the in vitro effects of prolactin and a dopamine D-2 receptor antagonist on the peritoneal activity of macrophages from primigravid and multigravid female rats during the third trimester of pregnancy. Oxidative bursts and phagocytosis in peritoneal macrophages were evaluated by flow cytometry. Primigravid and multigravid Wistar rats, during the third trimester of pregnancy (i.e., days 17-21), were used. Peritoneal fluid samples from these rats were first incubated with prolactin (10 and 100 nM) for different periods of time. The same procedure was repeated to evaluate the effects of domperidone (10 and 100 nM) on macrophage activity. Our results showed that macrophages from multigravid rats responded more effectively to in vitro incubation with prolactin, especially with regard to the intensity and percentage of phagocytosis. Additionally, these effects were more pronounced after incubation periods of 30 min or 4 h. These data suggest that macrophages during a second pregnancy become more sensitive to the phagocytotic effects of prolactin. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/04224-0 - Efeitos da administração de interleucina-2 no comportamento maternal e na liberação in vivo de dopamina em núcleo accumbens de ratas
• Beneficiário: Luciano Freitas Felicio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/61553-3 - Acoes da prolactina e da dopamina na atividade de macrofagos: modulacao pela experiencia reprodutiva em femeas.
• Beneficiário: Maria Isabel Roth de Carvalho Freitas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/51886-3 - Neuroimunomodulação: fármacos, estresse e citocinas nas relações entre os sistemas nervoso, endócrino e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático