Bacteriological analysis of necrotic pulp and fistulae in primary teeth

Autor(es)

Antônio Scalco FABRIS ^[1] ; Viviane NAKANO ^[2] ; Mario Júlio AVILA-CAMPOS ^[3]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology - Brasil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Anaerobe Lab, BR-05508900 Sao Paulo - Brazil

^[3] University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 22, n. 2, p. 118-124, 2014-04-00.

Resumo

Objectives: Primary teeth work as guides for the eruption of permanent dentition, contribute for the development of the jaws, chewing process, preparing food for digestion, and nutrient assimilation. Treatment of pulp necrosis in primary teeth is complex due to anatomical and physiological characteristics and high number of bacterial species present in endodontic infections. The bacterial presence alone or in association in necrotic pulp and fistula samples from primary teeth of boys and girls was evaluated. Material and Methods: Necrotic pulp (103) and fistula (7) samples from deciduous teeth with deep caries of 110 children were evaluated. Bacterial morphotypes and species from all clinical samples were determined. Results: A predominance of gram-positive cocci (81.8%) and gram-negative coccobacilli (49.1%) was observed. In 88 out of 103 pulp samples, a high prevalence of Enterococcus spp. (50%), Porphyromonas gingivalis (49%), Fusobacterium nucleatum (25%) and Prevotella nigrescens (11.4%) was observed. Porphyromonas gingivalis was detected in three out of seven fistula samples, Enterococcus spp. in two out of seven samples, and F. nucleatum, P. nigrescens and D. pneumosintes in one out of seven samples. Conclusions: Our results show that Enterococcus spp. and P. gingivalis were prevalent in necrotic pulp from deciduous teeth in boys from 2 to 5 years old, and that care of the oral cavity of children up to five years of age is important. (AU)

• Processo FAPESP: 08/57330-4 - Expressão dos genes bmeABC de bombas de efluxo de Bacteroides fragilis em Escherichia coli, caracterização do perfil de substrato das bombas bmeABC e sua relação com a resistência aos antimicrobianos
• Beneficiário: Viviane Nakano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/58738-7 - Citotoxicidade, aderência e invasão de cepas de Aggregatibacter actinomycetemcomitans isolados de pacientes portadores e não portadores de doença periodontal
• Beneficiário: Mario Júlio Ávila-Campos
• Modalidade de apoio: Auxílio à Pesquisa - Regular