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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A Rapid and Simple Method for Determination of 5-Hydroxytryptophan in Dietary Supplements by Capillary Electrophoresis

Texto completo
Autor(es):
Coelho, Aline G. [1] ; Aguiar, Fernanda P. C. [1] ; de Jesus, Dosil P. [1, 2]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of the Brazilian Chemical Society; v. 25, n. 4, p. 783-787, APR 2014.
Citações Web of Science: 7
Resumo

A rapid and simple capillary electrophoresis (CE) method was developed to determine 5-hydroxytryptophan (5-HTP) in samples of commercial dietary supplements. The optimized background electrolyte (BGE) was composed by 20 mmol L-1 sodium phosphate (pH 10) and 0.2 mmol L-1 cetyltrimethylammonium bromide (CTAB) for electroosmotic flow inversion. The limits of detection (LOD) and quantification (LOQ) for 5-HTP were 3.1 and 10.1 mu mol L-1, respectively. The analytical curve demonstrated linearity over a concentration range of 0-500 mu mol L-1 with a correlation coefficient (R-2) of 0.9995. The intraday instrumental precision (repeatability) as relative standard deviation (RSD) in terms of peak area and migration time of the 5-HTP were 2.2 and 1.9% (n = 8), respectively. The CE method was applied to the analysis of four different samples of commercial dietary supplements and the RSD (n = 3) for the determinations ranged from 2 to 3%. The accuracy of the method was evaluated comparing the measured 5-HTP concentrations to those obtained by a high performance liquid chromatography (HPLC) method. The relative error ranged from 0 to 5.9% (n = 3) and no significant differences between the concentrations were observed at a confidence level of 95%. (AU)

Processo FAPESP: 08/53868-0 - Síntese e caracterização de novos materiais estrategicamente estruturados e organizados para aplicações em dispositivos analíticos e fotônicos
Beneficiário:Lauro Tatsuo Kubota
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
Beneficiário:Lauro Tatsuo Kubota
Linha de fomento: Auxílio à Pesquisa - Temático