TLR2-and 4-independent immunomodulatory effect of high molecular weight components from Ascaris suum

Autor(es)

Favoretto, Bruna C. ^[1] ; Silva, Sandriana R. ^[2] ; Jacysyn, Jacqueline F. ^[3] ; Camara, Niels O. S. ^[4] ; Faquim-Mauro, Eliana L. ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Inst Pasteur, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med LIM62, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Immunology; v. 58, n. 1, p. 17-26, MAR 2014.
• Citações Web of Science: 4

Resumo

Components of high molecular-weight (PI) obtained from Ascaris suum extract down-regulate the Th1/Th2-related immune responses induced by ovalbumin (OVA)-immunization in mice. Furthermore, the PI down-modulates the ability of dendritic cells (DCs) to activate T lymphocytes by an IL-10-mediated mechanism. Here, we evaluated the role of toll like receptors 2 and 4 (TLR2 and 4) in the modulatory effect of PI on OVA-specific immune response and the PI interference on DC full activation. An inhibition of OVA-specific cellular and humoral responses were observed in wild type (WT) or in deficient in TLR2 (TLR2(-/-)) or 4 (TLR4(-/-)) mice immunized with OVA plus PI when compared with OVA-immunized mice. Low expression of class II MHC, CD40, CD80 and CD86 molecules was observed in lymph node (LN) cells from WT, TLR2(-/-) or TLR4(-/-) mice immunized with OVA plus PI compared with OVA-primed cells. We also verified that PI was able to modulate the activation of DCs derived from bone marrow of WT, TLR2(-/-) or TLR4(-/-) mice induced in vitro by agonists of TLRs, as observed by a decreased expression of class II MHC and costimulatory molecules and by low secretion of pro-inflammatory cytokines. Its effect was accompanied by IL-10 synthesis. In this sense, the modulatory effect of PI on specific-immune response and DC activation is independent of TLR2 or TLR4. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/10393-1 - Estudo da participação dos receptores DC-SIGN e MR nos mecanismos de supressão da resposta imune induzida por componentes de alta massa molecular do extrato de Ascaris suum
• Beneficiário: Bruna Cristina Favoretto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/23735-0 - Mecanismos envolvidos na modulação da resposta imune induzidos por antígenos de alta massa molecular do extrato de Ascaris suum e crotoxina isolada do veneno de Crotalus durissus terrificus
• Beneficiário: Eliana Faquim de Lima Mauro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/56883-7 - Avaliacao da participacao dos receptores do tipo toll e de lectina tipo c na supressao da resposta imune anti ovalbumina induzida por componentes de alta massa molecular do extrato de ascaris suum.
• Beneficiário: Bruna Cristina Favoretto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/04201-2 - Estudo dos mecanismos de supressão da resposta imune induzida por componentes de Ascaris suum e crotoxina do veneno de Crotalus durissus terrificus: modulação da atividade funcional de células apresentadoras de antígeno
• Beneficiário: Eliana Faquim de Lima Mauro
• Modalidade de apoio: Auxílio à Pesquisa - Regular