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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

TLR2-and 4-independent immunomodulatory effect of high molecular weight components from Ascaris suum

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Author(s):
Favoretto, Bruna C. [1] ; Silva, Sandriana R. [2] ; Jacysyn, Jacqueline F. [3] ; Camara, Niels O. S. [4] ; Faquim-Mauro, Eliana L. [1]
Total Authors: 5
Affiliation:
[1] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil
[2] Inst Pasteur, Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med LIM62, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Molecular Immunology; v. 58, n. 1, p. 17-26, MAR 2014.
Web of Science Citations: 4
Abstract

Components of high molecular-weight (PI) obtained from Ascaris suum extract down-regulate the Th1/Th2-related immune responses induced by ovalbumin (OVA)-immunization in mice. Furthermore, the PI down-modulates the ability of dendritic cells (DCs) to activate T lymphocytes by an IL-10-mediated mechanism. Here, we evaluated the role of toll like receptors 2 and 4 (TLR2 and 4) in the modulatory effect of PI on OVA-specific immune response and the PI interference on DC full activation. An inhibition of OVA-specific cellular and humoral responses were observed in wild type (WT) or in deficient in TLR2 (TLR2(-/-)) or 4 (TLR4(-/-)) mice immunized with OVA plus PI when compared with OVA-immunized mice. Low expression of class II MHC, CD40, CD80 and CD86 molecules was observed in lymph node (LN) cells from WT, TLR2(-/-) or TLR4(-/-) mice immunized with OVA plus PI compared with OVA-primed cells. We also verified that PI was able to modulate the activation of DCs derived from bone marrow of WT, TLR2(-/-) or TLR4(-/-) mice induced in vitro by agonists of TLRs, as observed by a decreased expression of class II MHC and costimulatory molecules and by low secretion of pro-inflammatory cytokines. Its effect was accompanied by IL-10 synthesis. In this sense, the modulatory effect of PI on specific-immune response and DC activation is independent of TLR2 or TLR4. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 08/04201-2 - Study of the mechanism of immunosuppression induced by Ascaris suum components and crotoxin isolated from Crotalus durissus terrificus venom: modulation of functional activity of antigen presenting cells
Grantee:Eliana Faquim de Lima Mauro
Support type: Regular Research Grants
FAPESP's process: 11/23735-0 - Mechanisms involved in the modulation of the immune response induced by high molecular weight components of Ascaris suum extract and crotoxin isolated from Crotalus durissus terrificus venom
Grantee:Eliana Faquim de Lima Mauro
Support type: Regular Research Grants
FAPESP's process: 10/10393-1 - Study of the involvement of DC-SIGN and MR receptors in the mechanisms of immunesuppression induced by high molecular weight components of Ascaris suum extract
Grantee:Bruna Cristina Favoretto
Support type: Scholarships in Brazil - Doctorate