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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)


Texto completo
Nagaishi, V. S. [1] ; Cardinali, L. I. [1] ; Zampieri, T. T. [1] ; Furigo, I. C. [1] ; Metzger, M. [1] ; Donato, Jr., J. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Neuroscience; v. 259, p. 71-83, FEB 14 2014.
Citações Web of Science: 34

Rodents exhibit leptin resistance and high levels of prolactin/placental lactogens during pregnancy. A crosstalk between prolactin and leptin signaling has been proposed as a possible mechanism to explain the changes in energy balance during gestation. However, it remains unclear if specific neuronal populations co-express leptin and prolactin receptors. Therefore, our present study was undertaken to identify in the mouse brain prolactin-responsive cells that possibly express the leptin receptor (LepR). In addition, we assessed the leptin response in different brain nuclei of pregnant and nulliparous mice. We used a LepR-reporter mouse to visualize LepR-expressing cells with the tdTomato fluorescent protein. Prolactin-responsive cells were visualized with the immunohistochemical detection of the phosphorylated form of the signal transducer and activator of transcription-5 (pSTAT5-ir). Notably, many neurons that co-expressed tdTomato and pSTAT5-ir were observed in the medial preoptic area (MPA, 27-48% of tdTomato cells), the retrochiasmatic area (34-51%) and the nucleus of the solitary tract (NTS, 16-24%) of prolactin-treated nulliparous mice, pregnant mice and prolactin-treated leptin-deficient (ob/ob) mice. The arcuate nucleus of the hypothalamus (8-22%), the medial tuberal nucleus (11-15%) and the ventral premammillary nucleus (4-10%) showed smaller percentages of double-labeled cells among the groups. Other brain nuclei did not show significant percentages of neurons that co-expressed tdTomato and pSTAT5-ir. Late pregnant mice exhibited a reduced leptin response in the MPA and NTS when compared with nulliparous mice; however, a normal leptin response was observed in other brain nuclei. In conclusion, our findings shed light on how the brain integrates the information conveyed by leptin and prolactin. Our results corroborate the hypothesis that high levels of prolactin or placental lactogens during pregnancy may directly interfere with LepR signaling, possibly predisposing to leptin resistance. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/18086-0 - Bases moleculares da resistência à leptina
Beneficiário:Jose Donato Junior
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 12/02388-3 - Topografia e assinatura neuroquímica das projeções entre a habenula lateral, o núcleo rostromedial tegmental e o núcleo dorsal da rafe no rato
Beneficiário:Martin Andreas Metzger
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/15517-6 - Fatores moleculares envolvidos nas alterações metabólicas durante a gestação: papel do SOCS3
Beneficiário:Thais Tessari Zampieri
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/16374-7 - Interação entre leptina e prolactina N‚O encéfalo: possível mecanismo dè alterações metabólicas ná gravidez
Beneficiário:Vanessa Sayuri Nagaishi
Linha de fomento: Bolsas no Brasil - Iniciação Científica