Laser and light-emitting diode effects on pre-osteoblast growth and differentiation

Autor(es)

Pagin, Marina Tochetti ^[1] ; de Oliveira, Flavia Amadeu ^[2] ; Oliveira, Rodrigo Cardoso ^[2] ; Passanezi Sant'Ana, Adriana Campos ^[3] ; Rubo de Rezende, Maria Lucia ^[3] ; Aguiar Greghi, Sebastio Luiz ^[3] ; Damante, Carla Andreotti ^[3]

Afiliação do(s) autor(es)

^[1] Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil

^[2] Bauru Sch Dent, Dept Biol Sci, BR-17012901 Sao Paulo - Brazil

^[3] Bauru Sch Dent, Discipline Periodont, BR-17012901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 29, n. 1, p. 55-59, JAN 2014.
• Citações Web of Science: 21

Resumo

The acceleration of bone regeneration by low-intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. The purpose of this study is to compare the effects of light-emitting diode (LED) and laser on pre-osteoblast MC3T3 proliferation and differentiation. Cells were irradiated with red, infrared, and LED (3 and 5 J/cm(2)). Lasers had a power density of 1 W/cm(2) and irradiation time of 2 and 5 s. LED had a power density of 60 mW/cm(2) and irradiation time of 50 and 83 s. Control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, and 96 h), and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72 and 96 h and 7 and 14 days). At 24 h, the cell growth was enhanced 3.6 times by LED (5 J/cm(2)), 6.8 times by red laser (3 J/cm(2)), and 10.1 times by red laser (5 J/cm(2)) in relation to control group (p < 0.05). At the other periods, there was no influence of irradiation on cell growth (p > 0.05). The production of ALP was not influenced by irradiation at any period of time (p > 0.05). Low-intensity laser and LED have similar effects on stimulation of cell growth, but no effect on cell differentiation. (AU)

• Processo FAPESP: 10/18361-1 - Avaliação comparativa dos efeitos da fototerapia com laser e LED na proliferação, metabolismo e diferenciação de pré-osteoblastos
• Beneficiário: Marina Tochetti Pagin
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica