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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of Nigrostriatal Neurodegeneration and Neuroinflammation Following Repeated Intranasal 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP) Administration in Mice, an Experimental Model of Parkinson's Disease

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Tristao, Fabrine S. M. [1] ; Amar, Majid [1] ; Latrous, Ines [1] ; Del-Bel, Elaine A. [2, 3] ; Prediger, Rui D. [4] ; Raisman-Vozari, Rita [1, 5, 6, 7]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] CRICM, UMR 975, INSERM, Paris - France
[2] Univ Sao Paulo, Dept MEF Fisiol, Fac Odontol Ribeirao Preto, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, NAPNA, BR-09500900 Sao Paulo - Brazil
[4] Univ Fed Santa Catarina, Dept Farmacol, Ctr Ciencias Biol, Florianopolis, SC - Brazil
[5] Univ Paris 06, Fac Med, Paris - France
[6] CNRS, UMR, F-7225 Paris - France
[7] Hop La Pitie Salpetriere, Inst Cerveau & Moelle Epiniere, F-75651 Paris 13 - France
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: NEUROTOXICITY RESEARCH; v. 25, n. 1, p. 24-32, JAN 2014.
Citações Web of Science: 13

Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting approximately 1 % of the population older than 60 years. The administration of the proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice is the most widely used approach to elucidate the mechanisms of cell death involved in PD. However, the magnitude of the PD-like neurodegeneration induced by MPTP depends on many variables, including the regimen of its administration. It has been demonstrated that intranasal (i.n.) administration of MPTP constitutes a new route of toxin delivery to the brain that mimics environmental exposure to neurotoxins. Previous data showed that mice submitted to chronic and acute i.n. MPTP treatment displayed a robust (similar to 80 %) and moderate (similar to 55 %) loss of striatal dopamine, respectively. However, little is known about the neurodegenerative and neuroinflammatory processes following a subacute i.n. MPTP administration in mice. Here, the C57BL/6 mice were infused intranasally with MPTP (1 mg/nostril/day) during 4 consecutive days. At 7 and 28 days after the last administration, the subacute i.n. MPTP regime decreased the tyrosine hydroxylase (TH)-labeling in the striatum (40-50 %) and substantia nigra (25-30 %) and increased the astrogliosis in such brain areas at both time points. Taken together, our data showed that the subacute administration of MPTP into the nasal cavity of C57BL/6 mice induces long-lasting neurodegeneration and neuroinflammation in the nigrostriatal pathway, thus representing a valuable animal model for the investigation of neuroprotective strategies in PD. (AU)

Processo FAPESP: 12/17626-7 - Mecanismos celulares e moleculares envolvidos no papel de neurotransmissores atípicos em transtornos neuropsiquiátricos
Beneficiário:Francisco Silveira Guimaraes
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/55092-9 - Analysis of nitric oxide synthase in dopaminergic and non dopaminergic neurons in experimental Parkinson Disease: role of NOS inhibitors in L-DOPA induced diskinesias
Beneficiário:Elaine Aparecida Del Bel Belluz Guimarães
Linha de fomento: Auxílio à Pesquisa - Regular