Chromosomal characteristics of a Brazilian whip spider (Amblypygi) and evolutionary relationships with other arachnid orders

Autor(es)

Paula-Neto, E. ^[1] ; Araujo, D. ^[2] ; Carvalho, L. S. ^[3] ; Cella, D. M. ^[1] ; Schneider, M. C. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Biol, Rio Claro, SP - Brazil

^[2] Univ Fed Mato Grosso do Sul, Ctr Ciencias Biol & Saude, Setor Biol Geral, Campo Grande, MS - Brazil

^[3] Univ Fed Piaui, Floriano, PI - Brazil

^[4] Univ Fed Sao Paulo, Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 12, n. 3, p. 3726-3734, 2013.
• Citações Web of Science: 3

Resumo

We analyzed mitotic and meiotic cells of a Brazilian amblypygid, Heterophrynus longicornis, using conventional and molecular cytogenetic techniques (Giemsa staining, C-banding, Ag-NOR, and FISH with rDNA probe). This is the first study that focuses solely on amblypygid chromosomes; it was undertaken to add data on cytogenetic knowledge of this group and contribute to the understanding of chromosome evolution in the Arachnida. We found 2n = 66 for male and female individuals, monocentric chromosomes, and absence of morphologically differentiated sex chromosomes. C-banding showed heterochromatin in the pericentromeric region of most chromosomes. Mitotic and meiotic nuclei submitted to silver impregnation and FISH revealed, respectively, Ag-NORs and ribosomal genes in the terminal region of two chromosome pairs. Most chromosome features that we observed in H. longicornis are shared with species of other arachnid orders; however, the absence of morphologically differentiated sex chromosomes in amblypygid contrasts with the remarkable variety of sex chromosome systems recorded for the Araneae. Consequently, we conclude that analysis of species of the Tetrapulmonata clade is useful for understanding the trends of sex chromosome evolution in this arachnid group. (AU)

• Processo FAPESP: 10/14193-7 - Evolução cromossômica em aranhas caranguejeiras (Mygalomorphae) e haploginas basais (Araneomorphae)
• Beneficiário: Emygdio de Paula Neto
• Modalidade de apoio: Bolsas no Brasil - Mestrado