Methylobacterium-plant interaction genes regulated by plant exudate and quorum sensing molecules

Autor(es)

Dourado, Manuella Nobrega ^[1] ; Bogas, Andrea Cristina ^[1] ; Pomini, Armando M. ^[2] ; Andreote, Fernando Dini ^[3] ; Quecine, Maria Carolina ^[1] ; Marsaioli, Anita J. ^[4] ; Araujo, Welington Luiz ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Piracicaba, SP - Brazil

^[2] Univ Estadual Maringa, Dept Quim, Maringa, Parana - Brazil

^[3] Univ Sao Paulo, Dept Ciencias Solos, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[4] Univ Estadual Campinas, Inst Quim, Campinas, SP - Brazil

^[5] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 44, n. 4, p. 1331-1339, OCT-DEC 2013.
• Citações Web of Science: 17

Resumo

Bacteria from the genus Methylobacterium interact symbiotically (endophytically and epiphytically) with different plant species. These interactions can promote plant growth or induce systemic resistance, increasing plant fitness. The plant colonization is guided by molecular communication between bacteria-bacteria and bacteria-plants, where the bacteria recognize specific exuded compounds by other bacteria (e.g. homoserine molecules) and/or by the plant roots (e.g. flavonoids, ethanol and methanol), respectively. In this context, the aim of this study was to evaluate the effect of quorum sensing molecules (N-acyl-homoserine lactones) and plant exudates (including ethanol) in the expression of a series of bacterial genes involved in Methylobacterium-plant interaction. The selected genes are related to bacterial metabolism (mxaF), adaptation to stressful environment (crtI, phoU and sss), to interactions with plant metabolism compounds (acdS) and pathogenicity (patatin and phoU). Under in vitro conditions, our results showed the differential expression of some important genes related to metabolism, stress and pathogenesis, thereby AHL molecules up-regulate all tested genes, except phoU, while plant exudates induce only mxaF gene expression. In the presence of plant exudates there is a lower bacterial density (due the endophytic and epiphytic colonization), which produce less AHL, leading to down regulation of genes when compared to the control. Therefore, bacterial density, more than plant exudate, influences the expression of genes related to plant-bacteria interaction. (AU)

• Processo FAPESP: 05/53748-6 - Interação entre bactéria endofítica expressando genes heterólogos e cana-de-açúcar geneticamente modificada
• Beneficiário: Maria Carolina Quecine Verdi
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/07594-5 - Identificação e caracterização de genes diferencialmente expressos na interação entre Methylobacterium mesophilicum e a planta hospedeira
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular