| Texto completo | |
| Autor(es): |
Caetano, L. C.
[1]
;
Gennaro, F. G. O.
[1]
;
Coelho, K.
[1]
;
Araujo, F. M.
[2]
;
Vila, R. A.
[1]
;
Araujo, A.
[1]
;
Bernardo, A. de Melo
[3]
;
Marcondes, C. R.
[4]
;
Lopes, S. M. Chuva de Sousa
[3]
;
Ramos, E. S.
[2, 1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Ginecol & Obstet, Ribeirao Preto, SP - Brazil
[3] Leiden Univ, Med Ctr, Dept Anat & Embryol, Leiden - Netherlands
[4] Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Genetics and Molecular Research; v. 13, n. 1, p. 838-849, 2014. |
| Citações Web of Science: | 8 |
| Resumo | |
The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of candidate genes implicated in vertebrate sex determination and differentiation were studied during embryonic chicken gonadal development. Gene expression profiles were obtained before, during, and after gonadal sex differentiation in females and males for DMRT1, SOX3, SOX9, DAX1, SCII, HINTZ, HINTW, and the male hypermethylated (MHM) region. Transcripts for the HINTZ, DMRT1, DAX1, SCII, and SOX9 genes were observed in both sexes, but expression was higher in male gonads and may be correlated with testicular differentiation. The expression patterns of HINTW, SOX3, and MHM suggest that they may act in ovary development and may be involved in meiosis entry. MHM was upregulated and DMRT1 was downregulated in females at the same developmental stage. This may indicate a regulation of DMRT1 by MHM ncRNA. Similar dynamics were observed between HINTW and HINTZ. This study reports on the MHM expression profile during gonadal development and its correlation with the expression of genes involved in vertebrate sex determination. (AU) | |
| Processo FAPESP: | 09/08313-2 - Expressão diferencial e padrão de metilação da região MHM durante o desenvolvimento gonadal de galinhas |
| Beneficiário: | Ester Silveira Ramos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |