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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential expression of the MHM region and of sex-determining-related genes during gonadal development in chicken embryos

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Author(s):
Caetano, L. C. [1] ; Gennaro, F. G. O. [1] ; Coelho, K. [1] ; Araujo, F. M. [2] ; Vila, R. A. [1] ; Araujo, A. [1] ; Bernardo, A. de Melo [3] ; Marcondes, C. R. [4] ; Lopes, S. M. Chuva de Sousa [3] ; Ramos, E. S. [2, 1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Ginecol & Obstet, Ribeirao Preto, SP - Brazil
[3] Leiden Univ, Med Ctr, Dept Anat & Embryol, Leiden - Netherlands
[4] Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Genetics and Molecular Research; v. 13, n. 1, p. 838-849, 2014.
Web of Science Citations: 8
Abstract

The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of candidate genes implicated in vertebrate sex determination and differentiation were studied during embryonic chicken gonadal development. Gene expression profiles were obtained before, during, and after gonadal sex differentiation in females and males for DMRT1, SOX3, SOX9, DAX1, SCII, HINTZ, HINTW, and the male hypermethylated (MHM) region. Transcripts for the HINTZ, DMRT1, DAX1, SCII, and SOX9 genes were observed in both sexes, but expression was higher in male gonads and may be correlated with testicular differentiation. The expression patterns of HINTW, SOX3, and MHM suggest that they may act in ovary development and may be involved in meiosis entry. MHM was upregulated and DMRT1 was downregulated in females at the same developmental stage. This may indicate a regulation of DMRT1 by MHM ncRNA. Similar dynamics were observed between HINTW and HINTZ. This study reports on the MHM expression profile during gonadal development and its correlation with the expression of genes involved in vertebrate sex determination. (AU)

FAPESP's process: 09/08313-2 - Methylation pattern and differential MHM region expression during chicken gonadal development
Grantee:Ester Silveira Ramos
Support Opportunities: Regular Research Grants